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Analysis of Coenzyme Q 10

School of Pharmacy:. School of Pharmacy:. Analysis of Coenzyme Q 10. By Taylor Sineath 6-1-01. Purpose. To test the qualities of Coenzyme Q10 dietary supplements using analytical methods. What is Coenzyme Q 10?.

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Analysis of Coenzyme Q 10

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  1. School of Pharmacy: School of Pharmacy: Analysis of Coenzyme Q 10 By Taylor Sineath 6-1-01

  2. Purpose To test the qualities of Coenzyme Q10 dietary supplements using analytical methods.

  3. What is Coenzyme Q 10? Naturally occurring compound found in organisms. Used for a variety of indications notably cardiovascular diseases.

  4. Forms/Dosage • GNC/FoodLion both 10 mg capsules • GNC is in a capsule form • FoodLion is in soft gel form

  5. Testing Methods • Weight variation • Disintegration • HPLC Assay

  6. Weight variation • Tests the variation in weight of capsules • Tests the deviation from the average • Tests the net fill of soft gel (FoodLion) • Tests the weight of the capsules (GNC)

  7. Disintegration • Tests the solubility in an environment supposed to imitate that of the stomach • Used 6 capsules in sodium acetate buffer • 37oC +/- 2oC • The limit was 45min

  8. HPLC Assay • Determine concentration of analyte within a sample • Measures it by light absorption • Analyzed 3 capsules per lot

  9. HPLC Setup • Used a wavelength of 275nm • Flow Rate 1ml/min • Column 150mm x 4.6mm Altima C18 3 • Mobile Phase (25:75) Hexanes:Methanol • Injection volume of 20 ul

  10. Sample Chromatogram

  11. Sample Preparation – Soft gels • Cut open the shell using forceps and a scalpel over a funnel in a 100ml volumetric flask • Wash the funnel,forceps and the scalpel into the 100ml flask with 20ml of hexanes • Sonicate for 5 min and diluted to volume with methanol • Then sonicate again for 5 min

  12. Sample Prep. (cont.) • Pipetted 2ml of the sample into a 50ml volumetric flask and diluted with methanol • Filled a HPLC vial 1/2 to 3/4 full

  13. Data from FoodLion Weight Variation

  14. Data From GNC Weight Variation

  15. Disintegration of FoodLion Brand

  16. Disintegration of GNC Brand

  17. Assay of FoodLion Soft gels

  18. Assay of GNC capsules

  19. Conclusions • Weight variations within brands consistent • Disintegrations within brands consistent • Assay would indicate variability within the lot and from lot to lot • Preliminary data suggests that FoodLion would be a better choice • Costs less $10 vs. $17 • 100 capsules vs. 90 capsule in bottle • Less variability

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