1 / 26

Plankton Culture for Feeding Larval Fish and Shellfish

Plankton Culture for Feeding Larval Fish and Shellfish. LaDon Swann Mississippi-Alabama Sea Grant Consortium Auburn University. Shellfish Larval Culture. Larval Rearing. Trocophore Size 50-60 microns 6-12 hours post-fertilization D-Hinge or Straight Hinge Size 70-80 microns

MikeCarlo
Télécharger la présentation

Plankton Culture for Feeding Larval Fish and Shellfish

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Plankton Culture for Feeding Larval Fish and Shellfish LaDon Swann Mississippi-Alabama Sea Grant Consortium Auburn University

  2. Shellfish Larval Culture

  3. Larval Rearing • Trocophore • Size 50-60 microns • 6-12 hours post-fertilization • D-Hinge or Straight Hinge • Size 70-80 microns • 24-48 hours post-fertilization • Bivalve Shell • Velum - Swimming and Feeding organ

  4. Larval Rearing • Veliger or Umbo Larvae • Size 80-300 microns • Stage last 8-12 days • Well defined umbo • Pediveliger or “Eyed” Larvae • Size >300 microns • Pigmented eye-spot • Muscular foot

  5. Phytoplankton Production • Feeding Larvae • Cell Size 4-8 microns • Species • Isochrysis galbana • Chaetoceros gracilis • Nannochloris sp. • Chlorella sp. • Pavlova lutheri

  6. Pavlova lutheri • Morphology • Golden brown • Spherical with 2 flagella • 3-6 µm • Salinity • 8-32 ppt • Temperature • 11-26 °C • Culture media • Guillards f/2 • Proximate Analysis • 52% Protein • 24% Carbs • 29% Fat

  7. Isochrysis galbana • Morphology • Tahiti (T-Iso strain) • Golden brown • Cells spherical with 2 flagella • 5-6 µm length, 2-4 µm wide • Salinity • 8-32 ppt • Temperature • 23 - 28°C • Culture media • Guillards f/2 • Proximate Analysis • 47% Protein • 24% Carbs • 17% Fat

  8. Chaetoceros gracilis • Morphology • Golden brown diatom • Medium-size 12 µm wide, 10.5 µm long • Cells united in chains • Salinity • 26 - 32 ppt • Temperature • 28 - 30°C • Culture media • Guillards f/2 with Si • Proximate Analysis • 28% Protein • 23% Carbs • 9% Fat

  9. Plankton for Shellfish • Broodstock and Spat • Cell Size 10-24 microns • Species • Tetraselmis sp. • Green • Thalassiosra sp. • Diatom

  10. Tetraselmis sp. • Morphology • Ovoid green cells • 14 to 23 µm L X 8 µm W • 4 flagella • Salinity • 28-36 ppt • Temperature • 22-26°C • Culture media • Guillards f/2 • Proximate Analysis • 55% Protein • 18% Carbs • 14% Fat

  11. Thalassiosra sp. • Morphology • Golden brown diatom • Cells united in chains • Barrel-shaped • Non-motile • 4 µm • Salinity • 26 – 32 ppt • Temperature • 22-29 °C • Culture media • Guillards f/2 with Si • Other characteristics

  12. Micro Algae Culture • Culture Water • Sterilization • Nutrient Enrichment • Inoculation • Cell Counts • Harvest and Feeding • Stock Culture

  13. Culture Water • Sources • Seawater • Saltwater wells • Prepared seawater • Salinity • 26-32 ppt

  14. Sterilization • Methods • Heat Pasteurization • 80 C and cool naturally • Autoclave • Sodium Hypochlorite (bleach) • 0.5 ml/L (10 drops) • Neutralize: 10-15 ml sodium thiosulfate (248 g/L) per liter • Hydrochloric acid (muriatic) • 0.2 ml/L (4 drops) • Neutralize: Na2CO3 0.4-0.9 g/L

  15. Nutrient Enrichment • Guillard’s f/2 • Part A and B • 0.5 ml/L each part • Na2Si03 for diatoms

  16. Inoculation • Culture vessels • 1,000 ml flask • 18.7 L (5 gal.) Carboy (glass) • 178 L (47 gal) Transparent Tank • Add enough algae to give a strong tint to the water • 100,000-200,000/ml • Lighting • Types • Sunlight • Fluorescent • VHO fluorescent • Metal halide • Highest Densities: 24/7

  17. Peak Algae Density I. Galbana 10-12 million cells/ml 10-14 days 2 wk stability T. pseudonana 4 million cells/ml 3 days 5 day stability Hemacytometer Count total in centermost 1 mm Multiply by 10,000 Product = number/ml Cell Counts Motile cells should be killed

  18. Algae Density Wk 1 = 50,000 cells/ml Wk 2+ = 100,000 cells/ml Onset of spatting = 200,000/ml Tank cleared in 24hrs Larvae Density 5-10 larvae/ml Harvest and Feeding Liters to feed = (TD x V)/CD TD = Target Density (1,000s/ml) V = Volume of larval tank (thousands of L) CD = Cell Density (millions/ml)

  19. Harvesting and Feeding • Batch • Total harvest occurs once or over several days • Semi-Continuous • Works well with diatoms • Part of the algae remains in the vessel • New media is added to replenish the algae removed

  20. Purchase pure strain Avoid contamination No aeration Half filed container Redundancy Holding Test tubes Conical flasks Transfer 1 drop/wk for T. pseudonana 1 drop/2 wk for I. galbana Stock Culture

  21. Marine Fish Larval Culture • Relies on zooplankton

  22. Marine Rotifer Brachionus plicatilis • Culture units • 40L plastic bags • 40L cone-bottomed tanks • Temperature 27-30 C • Salinity 26 ppt

  23. Rotifers • Laboratory production • 100 to 200+ mm size • 2-3 week life span • small size suitable as first food

  24. Feeding Marine Fish • Rotifers • Typical first food in hatchery • Feed algae or yeast • Enrichment needed

  25. Artemia • Feeding of older larvae

  26. Use of copepods

More Related