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High-Speed Fluorescence Polarization Measurements of Single Myosin V Molecules

High-Speed Fluorescence Polarization Measurements of Single Myosin V Molecules Dawn A. Bonnell, University of Pennsylvania, DMR 0425780. Some motions of myosin V are too fast to resolve by polTIRF. Tilting Angle (  ) and Rotational Diffusion (  ) of Myosin V Lever Arm. 90 . . 45 .

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High-Speed Fluorescence Polarization Measurements of Single Myosin V Molecules

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  1. High-Speed Fluorescence Polarization Measurements of Single Myosin V Molecules Dawn A. Bonnell, University of Pennsylvania, DMR 0425780 Some motions of myosin V are too fast to resolve by polTIRF Tilting Angle () and Rotational Diffusion () of Myosin V Lever Arm 90  45 4 ms  0 90   4 ms 45 0 sec 0 0.4 0.8 1.2 AR Dunn & JA Spudich, NSMB, 2007 • Time resolution increased 100x from 40 to 4 ms. • Researchers at the Nanoscale Science and Engineering Center (NSEC) at the University of Pennsylvania want to visualize novel myosin V motions between steps. Understanding Nature’s molecular motors may explain certain diseases and inspire man-made nano-machines.

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