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Improving RAST annotations within RAST

Improving RAST annotations within RAST. Veronika Vonstein Fellowship for Interpretation of Genomes December 2008. Your genome after RAST. SEED genome in RAST. Use Function based c omparison t ool. Choose genome to compare to.

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Improving RAST annotations within RAST

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  1. Improving RAST annotations within RAST Veronika Vonstein Fellowship for Interpretation of Genomes December 2008

  2. Your genome after RAST

  3. SEED genome in RAST

  4. Use Function based comparison tool

  5. Choose genome to compare to

  6. Compare Metabolic Reconstruction of your genome (A) and the SEED genome (B)

  7. 1. Case: Candidate present but Function different But to eliminate the risk of annotating a paralog

  8. Compare Regions

  9. Annotate Chromosomal Clusters

  10. Annotate set 1

  11. Set 1 done continue

  12. 2. Case: The real missing

  13. 3. Case: Missed gene call

  14. Your genome after RAST

  15. Your improved genome

  16. Summary • Re-annotated 490 gene functions • Increased the number of pegs in subsystems from 36% to 50% • This added 60 subsystems to the metabolic reconstruction of your genome • Possible to add missed genes, annotate them and connect them to subsystems

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