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Evaluation of Cloned DNA for B19 Genotypes

Evaluation of Cloned DNA for B19 Genotypes. Dr Jacqueline Fryer, Division of Virology NIBSC. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region. A6. Servant et al ., J. Virol. 2002. Plasmid Information. Evaluation of Plasmids.

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Evaluation of Cloned DNA for B19 Genotypes

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  1. Evaluation of Cloned DNA for B19 Genotypes Dr Jacqueline Fryer, Division of Virology NIBSC

  2. Genetic Diversity of Parvovirus B19: Analysis of the NS1/VP1 Region A6 Servant et al., J. Virol. 2002

  3. Plasmid Information

  4. Evaluation of Plasmids • Transformed each plasmid & maxi preps grown up for each clone • DNA sequence analysis of insert/vector region determined • Restriction enzyme digestions of clones • Analysis by consensus real-time PCR assay

  5. Analysis of Plasmid DNAs by Consensus Assay for Parvovirus B19

  6. Distribution and Use of Clones to OMCL Network and Manufacturers • To be distributed through the EDQM PTS for parvovirus B19 DNA • To be used for the validation of assays & material not to be distributed beyond the recipients laboratory • MTA with NIH for pB19-N8, pA6 • Hôpital Armand Trousseau permission to supply pV9 & pD91.1

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