The Polymerase Chain Reaction

1. The Polymerase Chain Reaction Some milestones In molecular biology recognised by the award of the Nobel prize

2. Table (continued)

3. Number of publications citing the polymerase chain reaction from 1985 to 1999

4. Klenow fragment used for DNA sequencing

5. Primers for DNA polymerase

6. Primer design

7. The use of PCR to amplify DNA moleculesin vitro

8. The basic polymerase chain reaction

9. The polymerase chain reaction

10. PCR amplification of DNA Fragment

11. The PCR cycle

12. The PCR leads to amplification of the target sequences

13. Mismatching primers can lead to the efficient amplification of undesired sequences

14. Cloning DNA using the PCR and the consequences of polymerase mistakes

15. Amplifying cloned DNA from vectors

16. Alu-PCR is used to isolate human-specific DNA sequences

17. Asymmetric PCR For producing single- stranded DNA for sequencing

18. Detecting cancer cells using the PCR

19. Determining sex of fetuses at risk for X-linked inherited disorders

20. Nested Primers increase the specificity of a PCR

21. Nested PCR

22. Gene linkage analysis using single sperm

23. Reverse transcriptase PCR (RT-PCR)

24. Competitor RT-PCR

25. Inverse PCR

26. Some variants of the basic PCR Process

27. Visualisation of PCR products