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Station 1: Introduction to the Microscope

Station 1: Introduction to the Microscope. Care Parts Focusing. Microscope Care. Always carry with 2 hands Do not force knobs Always store covered Keep objects clear of desk and cords Only use lens paper for cleaning. Microscope Parts. Eyepiece. Body Tube. Revolving Nosepiece. Arm.

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Station 1: Introduction to the Microscope

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  1. Station 1: Introduction to the Microscope Care Parts Focusing

  2. Microscope Care • Always carry with 2 hands • Do not force knobs • Always store covered • Keep objects clear of desk and cords • Only use lens paper for cleaning

  3. Microscope Parts Eyepiece BodyTube RevolvingNosepiece Arm ObjectiveLens Stage StageClips CoarseFocus Diaphragm FineFocus Light Base

  4. Using the Microscope • Place the Slide on the Microscope • Use Stage Clips • For low power, Click Nosepiece to the lowest (shortest) setting • Look into the Eyepiece • Use the Coarse Focus

  5. Using High Power • Follow steps to focus using low power • Click the nosepiece to the longest objective • Do NOTuse the Coarse Focusing Knob • Use the Fine Focus Knob to bring the slide in focus What can you find on your slide? Click here to Restart show for next group

  6. THE CELL

  7. Essential Questions • What defines Life? • What unifies all living things? • 3. Why are cells important? • 4. How is a cell related to other living things?

  8. What are you made of? Atoms and Elements??? correct, but rocks and pizza are made of atoms and elements too.

  9. What makes you different from those things? Organic Compounds Correct, but sugar and cow poop are made of organic compounds too.

  10. So a better question would be: What are you made of that makes you alive? Cells

  11. The most basic structure of any living thing, either an enormous whale or bacteria, is a cell.

  12. HISTORY OF THE CELL Most cells cannot be seen with the nakedeye. The discovery that cells make up all living things occurred after the invention of the microscope.

  13. Station 3 – Types of Microscopes and Magnification

  14. Electron microscope Dissecting microscope MICROSCOPES 1830’s

  15. MICROSCOPE TYPES Simple Microscope: • It is called simple because it has only 1 lens. Compound, Light Microscope: • It is called compound because it is composed of two lens systems.  • It uses light to view the object.

  16. ELECTRON MICROSCOPE: Uses electrons to gain data and view objects at extremely high magnifications.

  17. Scanning Electron Microscopes

  18. PHOTOS OF INSECTS USING AN ELECTRON MICROSCOPE

  19. Kangaroo sperm Mitochondrion http://www.uq.edu.au/nanoworld/images_1.html

  20. Transmission Electron Microscopes

  21. MICROSCOPE IMAGES Mold culture Dog lung alveolus

  22. Fish gill Baboon blood cell

  23. Nematode Velcro

  24. Butterfly Egg

  25. MAGNIFICATION Maximum magnification for compound light microscope: To find the magnification of a compound light microscope: MULTIPLY THE EYEPIECE LENS TIMES THE OBJECTIVE LENS! 400X the normal size of the specimen

  26. Examples: 300x 20x 250x

  27. Magnification • Do sample problems on your own in your packet. • MULTIPLY THE EYEPIECE LENS TIMES THE OBJECTIVE LENS!

  28. Household mold

  29. Station 4 Measure With A Microscope • Watch the video to demonstrate how to convert to microns (micrometers) http://www.showme.com/sh/?h=xqz9Wym

  30. How big are cells?

  31. Station 5 Microscope and Equipment Notes

  32. Staining • Used for: to see more detail • Problems: Usually kills the cell, Cannot observe live cells Unstained stained

  33. ELECTRON MICROSCOPE: Uses electrons to gain data and view objects at extremely high magnifications.

  34. Scanning Electron Microscopes

  35. PHOTOS OF INSECTS USING AN ELECTRON MICROSCOPE

  36. Striated Muscle Bacillus dividing

  37. Kangaroo sperm Mitochondrion http://www.uq.edu.au/nanoworld/images_1.html

  38. Bone Marrow (Colour Enhanced)

  39. Transmission Electron Microscopes

  40. Lyngbya majuscula (Fireweed) http://www.uq.edu.au/nanoworld/images_1.html

  41. Fish gill

  42. Carboniferous microspore Carboniferous miospore

  43. PHASE CONTRAST MICROSCOPE: • Is able to show contrast in transparent specimens (cells, thin tissue, organelles) • A way to look at living specimens without having to kill them • No staining is required

  44. ULTRACENTRIFUGE: Spins suspensions at high speeds to separate.

  45. MICRODISSECTION Uses a microscope to dissect very small organisms &/or their parts. Dissection of DNA from a nucleus of a cell.

  46. http://micro.magnet.fsu.edu/cells/animalcell.html

  47. Station 7

  48. Station 6 • Do on your own with your group or individually

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