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Holiday Concerts: Whatcom Chorale Sunday, Dec. 12 3pm and 7:30 pm First Congregational Church

Holiday Concerts: Whatcom Chorale Sunday, Dec. 12 3pm and 7:30 pm First Congregational Church Handel's Messiah. Bellingham Chamber Chorus Friday, December 10 8 pm PAC Concert Hall Bach Magnificat and a bunch of modern stuff. Upcoming Biochemistry Seminars. M November 29 4 pm SL 130.

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Holiday Concerts: Whatcom Chorale Sunday, Dec. 12 3pm and 7:30 pm First Congregational Church

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  1. Holiday Concerts: Whatcom Chorale Sunday, Dec. 12 3pm and 7:30 pm First Congregational Church Handel's Messiah

  2. Bellingham Chamber Chorus Friday, December 10 8 pm PAC Concert Hall Bach Magnificat and a bunch of modern stuff.

  3. Upcoming Biochemistry Seminars M November 29 4 pm SL 130 F December 3 4 pm SL 130 Structure, Function and Engineering of the Homing Endonuclease I-CreI Extra Credit!!!!

  4. Figure 15-20cX-Ray structure of bovine trypsin. (c) A drawing showing the surface of trypsin (blue) superimposed on its polypeptide backbone (purple). Page 519

  5. Table 15-4 A Selection of Serine Proteases. Page 516

  6. Example of “convergent” evolution.

  7. Figure 15-22 Relative positions of the active site residues in subtilisin, chymotrypsin, serine carboxypeptidase II, and ClpP protease. Page 521

  8. Figure 15-21 The active site residues of chymotrypsin. Page 520

  9. Figure 15-23 Catalytic mechanism ofthe serine proteases. Page 522

  10. General base Electrostatic stabilization Rate determining step (Michaelis complex) Scissile bond

  11. General base catalysis decomposition

  12. Water replaces new N terminal Now the whole process operates in reverse:

  13. Stable acyl-enzyme intermediate Attacking nucleophile? Leaving group?

  14. Figure 15-25a Transition state stabilization in the serine proteases. (a) The Michaelis complex. Page 524

  15. Figure 15-25b Transition state stabilization in the serine proteases. (b) The tetrahedral intermediate. Page 524

  16. Figure 15-26a X-Ray structures of porcine pancreatic elastase in complex with the heptapeptide BCM7 (YPFVEPI). (a) The complex at pH 5. Page 525

  17. Figure 15-26b X-Ray structures of porcine pancreatic elastase in complex with the heptapeptide BCM7 (YPFVEPI). (b) The complex at pH 9. Page 525

  18. Stryer Fig. 9.16 Site directed mutagenesis of subtilisin. Note the log scale. Mutations in the catalytic triad lead to a dramatic loss of activity

  19. Figure 15-27 Activation of trypsinogen to form trypsin. Page 527

  20. Figure 15-28 Activation of chymotrypsinogen by proteolytic cleavage. Page 528

  21. Substrate activation schemes Stryer Fig. 9.17 an 18

  22. Figure 15-8 The alternating NAG–NAM polysaccharide component of bacterial cell walls. Page 507

  23. Chapter 12: Lipids and Membranes

  24. C16 and C18 most common * *usually. Bacteria have some odd number chains

  25. Rarely conjugated rare

  26. Figures 12-1 and 12-9

  27. Lipid structures

  28. Much more reduced than CHO or protein-->high energy storage.

  29. Glycerolphospholipids See Table 12-2

  30. Table 12-2 Classes of Phospholipids

  31. Figures 12-4, 12-6 and 12-7 ganglioside

  32. GM2:sphingosine linked to 4 sugars Tay-Sachs Disease: GM2 degradation enzyme missing

  33. Figure 12-9

  34. Figure 12-12

  35. membrane fluidity is affected by temperature above transition temp. below transition temp. See Table 12-1

  36. Figure 12-16

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