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Step-Up Year 1 Practical Laboratory Session: Iron Analysis

Step-Up Year 1 Practical Laboratory Session: Iron Analysis . Iron (Fe) . Iron is vital for a number of physiological processes Occurs naturally in food sources Also occurs naturally in our water . Iron in our water . Should we be concerned?

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Step-Up Year 1 Practical Laboratory Session: Iron Analysis

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  1. Step-Up Year 1 Practical Laboratory Session: Iron Analysis

  2. Iron (Fe) Iron is vital for a number of physiological processes • Occurs naturally in food sources • Also occurs naturally in our water

  3. Iron in our water • Should we be concerned? • Iron in water is found in two forms:- Ferrous (Fe2+)- Ferric (Fe3+) Fe3 + 3H2O  Fe(OH)3 + 3H+

  4. Iron in our water • A range of solutions from no iron to high iron content • Ferrous iron in water has addedto it thiocyanate (SCN) Fe(SCN)(H20)5]2+

  5. Iron in our water • How do we decide what should be classed as ‘low’ or ‘high’ iron content? • Calibration Curve – our baseline to compare against

  6. Iron Analysis • Using a Spectrophotometer at a wavelength 490nm to measure Absorbance (nm) • Using the calibration curve, we can determine unknown Iron Concentrations

  7. Before you start • Read the protocol thoroughly • Always read the protocol before you start and follow H&S • Remember Beer-Lambert Law ? • AnyQuestions • Absorbance =ε.c.l • where ε = constant • c = concentration • l = path length through solution

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