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Week 6

Week 6. Team has split into Dry Lab and Wet Lab Dry Lab: Wet Lab: Erika, - James, Prudence, -Chris, Clinton, -Tom, Yanis, -Krupa,. Wet Lab. Two key tasks: DNA synthesis Protocols and Reagents. Promoter and RBS. Genes. Terminators. DNA Synthesis.

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Week 6

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  1. Week 6 Team has split into Dry Lab and Wet Lab Dry Lab: Wet Lab: • Erika, - James, • Prudence, -Chris, • Clinton, -Tom, • Yanis, -Krupa,

  2. Wet Lab Two key tasks: • DNA synthesis • Protocols and Reagents

  3. Promoter and RBS Genes Terminators DNA Synthesis

  4. Promoter and RBS Promoters Inducible 1.Phyper-spank 2.pXyl Constitutive 1.p43 2.pVeg Sigma B 1.pctc 2.pgsiB RBS =12 combinations 1.gsiB 2.spoVG

  5. Genes Genes Light+Motility 1.YtvA 2.EspE Signal Peptide 1.SacB 2.LipA Biomaterials 1.Elastin 2.EAK 16 Registry 1.Antibiotics 2.GFPmut3b =4 Combinations

  6. Terminators Insertion Sequence Terminators Registry 1.Double Terminator Insertion Sequence 1.PyrD 2.AmyE

  7. Protocols • Cloning, • Culturing, • Transformation, • Testing,

  8. Cloning

  9. Culturing and Transformation This week we are planning to test culturing and transformation using integration vector. If successful will linearise vector and test for linear integration

  10. Testing • Have defined testing Constructs. • This week need to work out testing protocols, • Microscope training on Thursday!

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