Cuticle brightness classes

1. A Supplemental Figure S7. Phenotyping the F2 progeny from the cross M82d x P15C12 . A, Visual scoring of fruit brightness performed on Mature Green fruits. B, Distribution of the F2 progeny for fruit brightness. C, Visual scoring of cuticle permeability to toluidine blue. Intensity of the blue coloration of the fruit surface was visually assessed for scoring fruit permeability. D, Distribution of the F2 progeny for cuticle permeability. E, Water loss in fruit kept for 6 weeks in ambient air at room temperature. Fresh weight of the fruit was measured initially and after the 6 weeks storage period. F, Distribution of the F2 progeny for water loss. Results are expressed as percentage of the initial mass of the fruit. G, Photographs of epidermal cells from fruits from the F2 progeny ranged in the different cutin width classes. Measurements were done as indicated in Supplemental Fig. S6 on fresh pealed outer epidermis of Red Ripe fruits. Twelve independents measures of the width of the cutinized cell wall were done (20x magnification). H, Distribution of the F2 progeny for cutin width. Red arrows indicate the values for M82d, P15C12 mutant and their F1 hybrid. class 1 class 2 class 3 M82d / P15C12 / F1 B Frequency Cuticlebrightness classes C class 1 class 2 class 3 class 4 class 5 class 6 F1 D M82d / P15C12 Frequency Cuticlepermeability classes E 80-100 class 60-80 class 40-60 class 20-40 class 0-20 class M82d / F1 F Frequency P15C12 Percentage of the initial mass G 35-40 class 25-30 class 15-20 class 5-10 class P15C12 H F1 Frequency M82d Cutinwidth