1 / 14

Si Nitride Chip.

Si Nitride Chip. 4.4 mm. 4.4 mm. Window.

arista
Télécharger la présentation

Si Nitride Chip.

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Si Nitride Chip. 4.4 mm 4.4 mm Window unmodified silicon nitride window/chip as supplied by Adam Hall (NorcadaNX4402x)window is 30nm nitride, it is 10-20um (these look like 20um) square in a frame of 4.4mmx4.4mm 200um thick Si http://www.norcada.com/nitride_window_xray.php - all image areas were intended to overlap or fall closely adjacent to window

  2. CO samples from Marshall -origami sample 1-4 Sample 1 contains 1.7 nM origami (12ng/ul) with no extra tail extenderSample 2 contains 1.7 nM origami (12ng/ul) plus 200nm tail extension at about 1:1 ratio Sample 3 contains 1.7 nM origami (12ng/ul) plus 1200nm tail extension at about 1:1 ratio with origami Sample 4 contains ~10nM origami with 5x extra staples - this is unpurified so should have longest shelf life samples returned by Adam Hall - pores and origami (AH1-4) or just pores (AH5a and 5b) AH1 5x5, 400 nm sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2 (this is the source of the sample trace and of the one AFM image I sent) AH2 5x5, 400 nm sep, origami sample 2, measured in 50 mMKCl w/ TE and MgCl2 AH3 5x5, ~2.5 um sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2 AH4 5x5, ~1.6 um sep, origami sample 1, measured in 1 M KCl w/ TE and MgCl2 AH5a no origami 400nm sep AH5b no origami 2.8um sep

  3. Adam Hall (AH) Samples 1, 2, and 5a. 400-nm nanopore spacing.

  4. Adam Hall (AH) Sample 4. 1.6-µm nanopore spacing. 20 µm

  5. Adam Hall (AH) Samples 4 1600-nm nanopore spacing. AH4 - unrinsed, as recieved

  6. Adam Hall (AH) Samples 4 1600-nm nanopore spacing. approx., location of new zoomed scan below AH4 -imaged on crystal - then Tanner rinsed surface -looked clean no more crystals - to be continued

  7. Adam Hall (AH) Samples 4 1600-nm nanopore spacing. AH4 -imaged on crystal - then Tanner rinsed surface -looked clean no more crystals – images follow AH4 - off window

  8. Adam Hall (AH) Samples 4 1600-nm nanopore spacing. AH4 - rinsed AH4 - off window

  9. Adam Hall (AH) Samples 4 1600-nm nanopore spacing. AH4 -rinsed AH4 - at window

  10. Adam Hall (AH) Samples 4 1600-nm nanopore spacing. AH4 rinsed AH4 - on window – no array visible

  11. Adam Hall (AH) Samples 4 1600-nm nanopore spacing. AH4 -rinsed AH4 - on window - tried for dead center of window

More Related