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Dox-Al Subsidiaries

Dox-Al Subsidiaries. Doxal Swiss. Doxal France. Doxal Iberica. Doxal Taiwan. Doxal Australia. Dox-Al Distributors. FOODS WHERE TOXIGENIC MOULDS AND THEIR TOXINS MAY BE FOUND. FOODS WHERE TOXIGENIC MOULDS AND THEIR TOXINS MAY BE FOUND. TARGET ORGANS OF SOME MYCOTOXINS.

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Dox-Al Subsidiaries

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  1. Dox-Al Subsidiaries Doxal Swiss Doxal France DoxalIberica Doxal Taiwan Doxal Australia

  2. Dox-Al Distributors

  3. FOODS WHERE TOXIGENIC MOULDS AND THEIR TOXINS MAY BE FOUND

  4. FOODS WHERE TOXIGENIC MOULDS AND THEIR TOXINS MAY BE FOUND

  5. TARGET ORGANS OF SOME MYCOTOXINS

  6. MAXIMUM LIMITS OF AFLATOXINSIN FOODS (ASIA) (µ/kg)

  7. Minimum aw for growth of toxigenic mould species

  8. Temperature range for growth of toxigenic mould species

  9. MYCOTOXIN CONCENTRATIONS IN COMPLETE FEED IN CHINA Wang et al. (2003) - Asian Pork Magazine

  10. MYCOTOXIN CONCENTRATIONS IN COMPLETE FEED IN EUROPE(600 samples from 12 countries) (in house data, 2004)

  11. INDIVIDUAL EFFECT OF AFLATOXIN IN RATS • 3.5mg aflatoxins (AF)kg of diet • Male rats of 3 week of age, for 28 days. • Body weight gains were reduced 28% • FCR –18% • Increased weights of both kidney, heart • Decreased serum concentrations of total protein, albumin and inorganic phosphorus. • Increased concentrations of creatinine. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  12. Effects of a Modified Hydrated Sodium Calcium Aluminosilicate on Mycotoxicosis in Rats • 2 groups: control and captex 0.2% • Male rats of 3 weeks age • Diets containing AF 5mg/kg diet Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  13. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  14. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  15. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  16. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  17. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  18. Effects of a Modified Hydrated Sodium Calcium Aluminosilicate on Mycotoxicosis caused by D.O.N. in Rats • 2 groups: control and Captex T2 0.2% • Male rats of 3 weeks age • DON at 2 mg/kg of diet Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  19. DUODENUM – NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  20. ILEUM - NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  21. KYDNEY-NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  22. LIVER - NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  23. STOMAC- NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  24. Effects of a Modified Hydrated Sodium Calcium Aluminosilicate (Captex T-2) on Mycotoxicosis in Rats • 2 groups: control and Captex T2 0.2% • Male rats of 3 weeks age • T2 at 5 mg/kg diet Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  25. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  26. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  27. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  28. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  29. Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  30. Effects of Captex T2 (Modified Hydrated Sodium Calcium Aluminosilicate enriched with Glucomannans and Chitinase) on Mycotoxicosis caused by Fumonisin in Rats • 2 groups: control and Captex T2 0.2% • Male rats of 3 weeks age • FB1 3 mg/kg of diet Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  31. ILEUM – NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  32. KYDNEY – NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  33. LIVER – NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  34. STOMAC – NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  35. DUODENUM – NO ALTERATIONS Vopato I. Bizzini B. -1998 Italian Project M.S.T./09/96

  36. Effects of CAPTEX T-2 on Aflatoxin toxicity in chickens (Gallus gallus) • 3 groups (180 birds each) • Standard feed and water ad libitum for 20 consecutive days • Group A: standard blank feed • Group B: standard feed contaminated by 3 ppm AFLATOXIN • Group C: standard feed contaminated by 3 ppm Aflatoxin and fortified by 0.3% CAPTEX T-2 Riemschneider J. Chen C.C.- Doxal Italia 's Scientific Panel

  37. RESULTS: Riemschneider J. Chen C.C.- Doxal Italia 's Scientific Panel

  38. RESULTS: Riemschneider J. Chen C.C.- Doxal Italia 's Scientific Panel

  39. RESULTS: Riemschneider J. Chen C.C.- Doxal Italia 's Scientific Panel

  40. RESULTS: Riemschneider J. Chen C.C.- Doxal Italia 's Scientific Panel

  41. DISCUSSION: It has been found that the addition of 3 ppm Aflatoxin to a standard feed for broilers, caused dramatic lesions to most of the organs of the chicks exposed to the toxin, without any protection (Group B). The lesions found in liver, kidney, spleen, thymus and bursa of Fabricius were significantly more severe in Group B than in Group C. In fact, in Group C, not only the lesions that were found were of less severity, but, in most of the cases were concentrated to one bird. Carcasses of birds of Group B showed clear signs of micro-haemorrhages and evident bruises, to the extent that 20 birds were rejected (11%) at Carcass grading. A1 grading in Group B was 28% lower than in Group A and 26% lower than in Group C. Feed consumption and the final body weight of group C were similar to the ones of Group A. Group B mortality was 5 times higher than in the other two Groups. Plasmatic levels of calcium were similar in Groups A and C, while Group B showed a remarkable decrease, thus confirming a direct influence of Aflatoxicosis on calcium metabolism. Riemschneider J. Chen C.C.- Doxal Italia 's Scientific Panel

  42. TOXINS BINDING CAPACITY OF THREE FEED ADDITIVESIN VITRO • Each mycotoxin was solved at the level of 50 microgram into 200 ml of methanol, and kept under gentle stirring throughout the test period, in a 250 ml volumetric flask. Seven replicates for each mycotoxin were prepared. • 100 mcg and 250mcg aliquots of each Clay, Bentonite and CAPTEX-T2 were solved into the flasks, and kept under gentle stirring for 20 minutes; one flask of each mycotoxins was left as blank. • After 20 minutes, small aliquots were collected from each volumetric flask and then assayed by High Performance Liquid Chromathgraphy, Column Kromasyl, fluorescence detector, excitation 274 nm, flow rate 1 mL/min, emission 440 nm. Dr. Gautieri and Dr. G. Colajanni

  43. Results obtained: Dr. Gautieri and Dr. G. Colajanni

  44. Results obtained: Dr. Gautieri and Dr. G. Colajanni

  45. Results obtained: Dr. Gautieri and Dr. G. Colajanni

  46. Results obtained: Dr. Gautieri and Dr. G. Colajanni

  47. TOXINS BINDING CAPACITY OF THREE FEED ADDITIVESIN VITRO • Each mycotoxin was solved at the level of 50 microgram into 200 ml of methanol, and kept under gentle stirring throughout the test period, in a 250 ml volumetric flask. Seven replicates for each mycotoxin were prepared. • 100 mcg and 250mcg aliquots of each MYCOSORB , TOXISORBand CAPTEX-T2 were solved into the flasks, and kept under gentle stirring for 20 minutes; one flask of each mycotoxins was left as blank. • After 20 minutes, small aliquots were collected from each volumetric flask and then assayed by High Performance Liquid Chromathgraphy, Column Kromasyl, fluorescence detector, excitation 274 nm, flow rate 1 mL/min, emission 440 nm. Dr F Pedrazzini and Dr M Portali

  48. Results obtained: Dr F Pedrazzini and Dr M Portali

  49. Results obtained: Dr F Pedrazzini and Dr M Portali

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