1 / 11

Stephanie Bachar Matt Luchette

Isolation of Rare Circulating Tumor Cells in Cancer Patients by Microchip Technology Toner, et al. 2007. Stephanie Bachar Matt Luchette. Background: Circulating Tumor Cells. CTCs: tumor cells shed into the blood stream Motivation for detection Study biology of metastasis

belita
Télécharger la présentation

Stephanie Bachar Matt Luchette

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Isolation of Rare Circulating Tumor Cells in Cancer Patients by Microchip TechnologyToner, et al. 2007 Stephanie Bachar Matt Luchette

  2. Background: Circulating Tumor Cells • CTCs: tumor cells shed into the blood stream • Motivation for detection • Study biology of metastasis • Diagnose, detect, monitor cancer in patients without invasive biopsy The bone marrow niche: habitat to hematopoietic and mesenchymal stem cells, and unwitting host to molecular parasites Y Shiozawa, A M Havens, K J Pienta and R S Taichman

  3. Motivation for the “CTC-Chip” • Shortcomings prior to “CTC-Chip” • Accuracy: 20-60% • Purity: 0.1% • Non-viable cells Photoacoustic detection of CTCs. Viator lab at University of Missouri http://web.missouri.edu/~viatorj/tumor.htm

  4. How to use the “CTC Chip" • Flow sample through CTC Chip • EpCAM coated microposts • Verify with markers Pressure Control Blood on Rocker CTC Chip Toner 2007 EpCAM Non-CTCs CTCs Micropost Cytokeratin CD45

  5. Instrument Design: in silico • Maximize CTCs detected • Flow Velocity • Shear Force • Vary micropost characteristics • Theoretical capture efficiency of 65% Toner 2007

  6. Laboratory Results • 1 target cell/10^9 blood cells • Purity of capture ~60% (competitors = 0.1%) •  Efficiency of capture ~65% (competitors = 35%) • Captured cells were viable and could be further manipulated Capture purity per type of cancer Toner 2007

  7. Clinical Results • Identified CTCs in 99% cancer pts (competitors =  20-60%) • no false positives • Change in number of CTCs correlated with clinical path • No pre-processing required Change in CTCs captured per mL (red) with lung cancer patient mirrors change in tumor size (blue) Toner 2007

  8. Concerns and next steps • How cells used afterwards? • How to mass produce chips? • Financially viable? • How reliable is EpCAM as a caner cell marker? • FDA approval Paper tree-branch glucose and protein assay for urine technologyreview.com

  9. Recap/Applications • New diagnostic and prognostic tool for cancer patients • Provide alternative to biopsies for areas with low resources • Use to isolate and further study CTCs MRI of 67-year old patient with pancreatic cancer with the pancreatic tumor indicated imagingeconomics.com

  10. Works Cited --imagingeconomics.com --technologyreview.com --http://web.missouri.edu/~viatorj/tumor.htm --The bone marrow niche: habitat to hematopoietic and mesenchymal stem cells, and unwitting host to molecular parasites Y Shiozawa, A M Havens, K J Pienta and R S Taichman

More Related