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The effects of Gasoline on Aiptasia

The effects of Gasoline on Aiptasia. Group 4: Joon Chung Jimmy Wesman Heather Hollembeak Sebastian Camacho Nauman Chandry Will Quartell. Background Information. Problem: Many boater’s leak fuel into the ocean which may harm coral reefs.

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The effects of Gasoline on Aiptasia

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  1. The effects of Gasoline on Aiptasia Group 4: Joon Chung Jimmy Wesman Heather Hollembeak Sebastian Camacho Nauman Chandry Will Quartell

  2. Background Information • Problem: Many boater’s leak fuel into the ocean which may harm coral reefs. • We want to simulate this occurrence in our tank to see if the symbiosis between aiptasia and zooxanthellae is disrupted

  3. Hypotheses • H0: The addition of gasoline will not affect zooxanthellae density in aiptasia. • HA: The addition of gasoline will reduce zooxanthellae density in aiptasia.

  4. Clarification Bleaching is a descriptive term

  5. Procedure • November 1- We added .64 ml of 87 octane gasoline to the tank and turned the filter off. (according to CEAA) • November 8- Originally supposed to add .64 ml more • November 15- Decide whether to add more gasoline and if so how much? (Take into account Thanksgiving Holiday)

  6. Procedure Continued • Cell count using hemocytometer • Chlorophyll extraction and measurement using acetone • Protein concentration using Bradford method

  7. Results

  8. Things to think about • Statistical Significance • Hawaiian Species • Unwanted Aiptasia • A mollusk walks up to a sea cucumber and http://www.youtube.com/watch?v=CSU83KfjcWE

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