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Codon usage table, with preferred codons shown in blue. ------------------------------------------------------------------------------ Phe F TTT 2 | Ser S TCT 1 | Tyr Y TAT 3 | Cys C TGT 0

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  1. Codon usage table, with preferred codons shown in blue ------------------------------------------------------------------------------ Phe F TTT 2 | Ser S TCT 1 | Tyr Y TAT 3 | Cys C TGT 0 TTC 46 | TCC 51 | TAC 35 | TGC 18 Leu LTTA 0 | TCA 0 | *** * TAA 0 | *** * TGA 0 TTG 3 | TCG 7 | TAG 0 | Trp W TGG 22 ------------------------------------------------------------------------------ Leu L CTT 3 | Pro P CCT 1 | His H CAT 4 | Arg R CGT 7 CTC 13 | CCC 28 | CAC 19 | CGC 30 CTA 1 | CCA 1 | Gln Q CAA 1 | CGA 1 CTG 36 | CCG 3 | CAG 32 | CGG 2 ------------------------------------------------------------------------------ Ile I ATT 6 | Thr T ACT 4 | Asn N AAT 2 | Ser S AGT 2 ATC 33 | ACC 41 | AAC 68 | AGC 34 ATA 1 | ACA 2 | Lys K AAA 1 | Arg R AGA 0 Met M ATG 20 | ACG 4 | AAG 36 | AGG 5 ------------------------------------------------------------------------------ Val V GTT 4 | Ala A GCT 7 | Asp D GAT 13 | Gly G GGT 11 GTC 33 | GCC 68 | GAC 52 | GGC 65 GTA 1 | GCA 6 | Glu E GAA 3 | GGA 24 GTG 34 | GCG 5 | GAG 29 | GGG 2 ------------------------------------------------------------------------------ Figure S1. Codon usage bias in amy2 gene of D. melanogaster and D. pseudoobscura. The counts are a sum over the codons present in both D. melanogaster and D. pseudoobscura. Synonymous codon usage is highly biased in amy2, with the preferred codons shown in blue. For example, codons that encode leucine (Leu: highlighted in yellow) are not uniformly used. The CTG codon is clearly preferred over all other codons that encode leucine, and the TTA codon is never used within the amy2gene.

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