1 / 10

Previously Bio308

Previously Bio308. Hypotheses for molecular basis of bipolar disorder Suggest problem lies in protein targeting How are proteins targeted and delivered?. Proteins made in cytosol. Sorting places proteins in membrane and in lumen of organelles.

breena
Télécharger la présentation

Previously Bio308

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Previously Bio308 • Hypotheses for molecular basis of bipolar disorder • Suggest problem lies in protein targeting • How are proteins targeted and delivered? Proteins made in cytosol Sorting places proteins in membrane and in lumen of organelles PM (and other) proteins use Sec or SRP mediated translocation to become inserted into the ER (and only the ER) After insertion non-ER proteins are sorted and delivered lumenal vs membrane proteins let’s building a bud http://www.udel.edu/Biology/Wags/histopage/empage/ebv/ebv10.gif

  2. Budding CBI 13.1 Clathrin Fig 17-58

  3. Coat Components Clathrin COPI COPII Identity determined by what the vesicle contains and it’s coat. http://userpage.chemie.fu-berlin.de/biochemie/aghaucke/clath.jpg

  4. Highly hydrophobic + charged - charged Hydroxylated Other Sar1p-GTP form exposes helix that anchors protein to ER surface by ‘floating’ with hydrophobic a.a. interacting with membrane core Budding II ER vesicle budding Sar1p N-terminal helix Amino Acid Key Drin, G, and B. Antonny (2005) News and Views: Helices sculpt membrane. Nature vol: 437

  5. Budding III ER vesicle budding Floating many Sar1p in top leaflet makes it ‘bigger’ than the bottom one. Results --> bulge that can more easily interact with coat proteins. Drin, G, and B. Antonny (2005) News and Views: Helices sculpt membrane. Nature vol: 437

  6. ER vesicle budding….fission Fission Ring of parallel helices at neck might aid fission. New data for ER; had seen a protein (epsin) help deform PM for clathrin coated vesicles. May suggest that using a helix to deform membrane is common mechanism for budding/fission

  7. Targeting/Docking: What happens after budding? How do vesicles dock with specific target membrane? http://dir2.nichd.nih.gov/nichd/cbmb/sob/in_vivo_dyn.html

  8. The SNARE hypothesis V-SNARE T-SNARE Role of p115 Role of Rab proteins retrograde Fig 17-59

  9. Synaptic vesicle fusion VAMP Syntaxin SNAP 25 Rab3a Synaptotagmin

  10. Moving in the other direction: endocytosis Types: Phagocytosis– specialized cells Pinocytosis– all cells Connection– perhaps the # of our receptor’s on PM is controlled by endocytosis Pinocytosis ‘problem’ rate of pinocytosis internalizes 100% of PM per hour ? (How can this be?)

More Related