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Suppression of MAPKAPK2 in mammalian hibernation

Suppression of MAPKAPK2 in mammalian hibernation Mitogen-activated protein kinase-activated protein kinase-2 in Richardson’s ground squirrels ( Spermophilus Richardsonii ). Christopher A. Dieni Margaret and Wallace McCain Postdoctoral Fellow. http://chrisdieni.com .

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Suppression of MAPKAPK2 in mammalian hibernation

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  1. Suppression of MAPKAPK2 in mammalian hibernation Mitogen-activated protein kinase-activated protein kinase-2 in Richardson’s ground squirrels (SpermophilusRichardsonii) Christopher A. Dieni Margaret and Wallace McCain Postdoctoral Fellow http://chrisdieni.com

  2. Hibernation-associated stresses and adaptations

  3. Known responses in hibernating Spermophilus muscle ? ?

  4. MAPKAPK2 isolated from hibernating vs. euthermic squirrels = ↓ ~63% • Even when assayed at lower temperature (10 °C), still similar decrease • When compounding physiology-mediated and temperature-mediated differences = ~6% of euthermic, warm-temperature value Abnous et al., 2012

  5. Are decreased activity levels between euthermic and hibernating squirrels the result of decreased protein levels? • No… total MAPKAPK2 protein remains the same • Are decreased activity levels due to post-translational modification? Abnous et al., 2012

  6. MAPKAPK2 MAPKAPK2 P Phosphatase MAPKAPK2 MAPKAPK2 Inactivate phosphatase Assay

  7. In vitro dephosphorylation reduces MAPKAPK2 activity to barely detectable in both cases Abnous et al., 2012

  8. MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 P P P P P P MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 MAPKAPK2 Euthermia Hibernation In vitro dephosphorylation

  9. MAPKAPK2 inactivation seemingly at odds with other trends

  10. Other targets of MAPKAPK2 MAPKAPK2 P Tyrosine hydroxylase Glycogen synthase Akt CREB αB-crystallin Hsp25/27 5-Lipooxygenase

  11. MAPKAPK2 targets - expected phosphorylation state changes during hibernation * Abnous, K., Dieni, C.A., Storey, K.B. (2008) Regulation of Akt during hibernation in Richardson’s ground squirrels. Biochim. Biophys. Acta 1780: 185-193.

  12. Future efforts

  13. Thank you! Abnous, K., Dieni, C.A., Storey, K.B. Suppression of MAPKAPK2 during mammalian hibernation. Cryobiology. In press. http://chrisdieni.com http://twitter.com/chrisdieni

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