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Satish Pradhan Dnyanasadhana College, Thane .

Satish Pradhan Dnyanasadhana College, Thane . Department of Chemistry T.Y.B.Sc. Analytical Chemistry Paper-IV Sem-VI ION EXCHANGE CHROMATOGRAPHY By Dr.Bhagure G.R. Contents. 3.2.1 Introduction, Types of Ion Exchangers and their examples Ideal properties of Resin.

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Satish Pradhan Dnyanasadhana College, Thane .

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  1. SatishPradhan Dnyanasadhana College, Thane. • Department of ChemistryT.Y.B.Sc. Analytical ChemistryPaper-IV Sem-VIION EXCHANGE CHROMATOGRAPHY By Dr.Bhagure G.R.

  2. Contents • 3.2.1 Introduction, Types of Ion Exchangers and their examples Ideal properties of Resin. • 3.2.2 Ion Exchange equilibria and mechanism, Selectivity coefficient and separation factor. • 3.2.3 Factors affecting separation of ions. • 3.2.4 Ion Exchange capacity and its determination. • 3.2.5 Applications of Ion Exchange Chromatography Preparation Of demineralised water, Separation of Lanthanides, Preparation of exact concentration of acid or base, Separation of amino acids . • 3.3 Size Exclusion Chromatography (SEC) (02L) • 3.3.1Introduction, Basic Principle, Applications of SEC

  3. 3.3 Size Exclusion Chromatography (SEC) (02L) • 3.3.1Introduction, Basic Principle, Applications of SEC

  4. ION EXCHANGE CHROMATOGRAPHYH+ Na+ • H+ R

  5. ION EXCHANGE CHROMATOGRAPHYH+ Cl- • OH- R

  6. Na+ H+

  7. The first ion exchangers were synthetic resins designed for Applications such as demineralization, water treatment, and recovery of ions from wastes. The first ion exchangers designed for use with biological substances were the cellulose ion exchangersdeveloped by Peterson and Sober. • Ion exchangers based on dextrin (Sephadex), followed by those based on agarose (Sepharose CL-6B) and cross-linked cellulose (DEAE Sephacel) were the first ion exchange matrices to combine a spherical form with high porosity, leading to improved flow properties and high capacities for macromolecules

  8. INTEGRAL PART

  9. R=QAS • Ex.R-(NCH3)3+Cl--

  10. R= Primary amino group or secondary amino group

  11. Factors affecting separation of Ions:

  12. Small size ion • Large size ion

  13. ἁ

  14. EXPERIMENTAL TECHNIQUE Water level Ion Exchange Resin Cotton Plug Or glass wool plug

  15. Terms involved

  16. R-H+ Cation Exchange Resin R-OH- Anion Exchange Resin OH- H+

  17. 2) Separation of Lanthanides:-

  18. 2) Separation of Lanthanides:- • 57 La • 58 Ce • 59 Pr • 60 Nd Lanthanide Contraction • 61 Pm • 62 Sm • 63 Eu • 64 Gd • 65 Tb • 66 Dy • 67 Ho • 68 Er • 69 Tm • 70 Yb • 71 Lu

  19. 2) Separation of Lanthanides:- In this column is packed with cation exchange resin of type R-H. When solution containing mixture of lanthanides is poured on the top of the hen following equilibrium takes place, 3R-H + Ln3+ ----------------R3 ____Ln3+ 3H+ <-------------- In case of lanthanides the ionic radii of tripositive ions decreases with increase in atomic number i.e. from lanthanum to lutecium. Hydration of ion increases with decrease in ionic size and therefore hydrated size of lanthnone ion increases with increase in atomic number. Lanthnone ion having small hydrated size( La) will strongly adsorb on the resin and vise versa (Lu). Hence lanthnone ion held by resin decreases from La to Lu. When HCL solution is passed as mobile phase separation of lanthanides takes place in reverse order of atomic number. Lutecium ion will separate out first where as Lanthanum ion separate at last. For better separation elution is carried out using ammonium Citrate buffer at pH 2.8 -3.4.

  20. Thank You For Kind Attention

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