Genetics PCB 3063 (Chem & Physics 145) Tu//Th-3:00-3:15 PCB 3063 Lab Capacity Class:115 Lab:24

1. Genetics PCB 3063 (Chem & Physics 145) Tu//Th-3:00-3:15 PCB 3063 Lab Capacity Class:115 Lab:24

2. Course Description • This is a required course to provide undergraduate students majoring in • Biology or Marine Biology with an in depth looks at the structure and • function of nucleic acids (DNA/RNA) during replication, transcription and • proteins translation. • Topics • --The basic principles of DNA replication, recombination, • chromosomal variations, heredity and population genetics, • --DNA transcription into RNA, • --mRNA translation to produce proteins. • --Gene expression in viruses, prokaryotes and eukaryotes.

3. Learning Goals (Objectives) ---To gain a solid knowledge on the chemical nature, structure and function of nucleic acids. ---To understand gene expression: prokaryotes, eukaryotes and viral. ---To become familiar with appropriate vocabulary, primary literature and methods used by scientists to study Genetics. 

4. M. Alejandro Barbieri Office: AHC1 318C E-mail:barbieri@fiu.edu Course Website: http://www.fiu.edu/~barbieri, click on teaching--Genetics. Office Hours: Tuesday: 11:00-12:00; Thursday 11:00-12:00 or by appointment ONLY. .

5. Course Materials Text (Required) Principles of GENETICS, 6 Ed. by P. Snustad (2012) ISBN: 978-0-470-90359--9 Additional readings will be posted on the website. Although these will be announced in class, students are responsible for checking the class web page regularly for announcements and assignments.  Students should bring copies of the additional material to class to help them participate in class discussion. 

6. Textbook

7. Attendance Attendance will not be taken but is encouraged. Students are expected to attend class regularly, to arrive on time, and to remain in class until the end of the class period. Class attendance will help you master the course material. Furthermore, exam questions may cover material not found in the text book. If you miss class, you will still be responsible for any material disseminated or work that is assigned or due in your absence.

8. Grading Policy Exams:  There will be four (4) exams, which will include material covered in lecture and appendix reading.  Although the exams are not cumulative per se, you may be asked to synthesize material from various units.  Exam questions may consist of any combination of multiple choices, fill in the blank, short answer, labeling of figures and matching. If exam dates (or the material covered on the exam) need(s) to be modified, students will be given at least one week's notice in class. Exams will be closed book/note. You will be asked to show “your Panther ID” card on exam day so be sure to bring it to class.

9. Exams: Four (4) exams. Each exam=25 points= 50 questions. (Maximum=100 points). Final exam=“ NO” “I CURVE GRADES” Extra credits: class assignments NO make-up exams will be given!

10. Grading Policy

11. =70% Grade Curving Exam1 Max: 25pts 70%:17.5pts Mean= 69% Exam1 Mean=69%+1% Exam2 Max: 25pts 70%:17.5pts Mean= 56% Exam2 Mean= 56%+14% Exam3 Max: 25pts 70%:17.5pts Mean= 47% Exam3 Mean= 47%+23% Exam4 Max: 25pts 70%:17.5pts Mean= 70% Exam4 Mean= 70%+0%

12. Exams 5/29/2012EXAM #1 6/19/2012 EXAM #2 7/10/2012EXAM #3 7/262012 EXAM #4

13. http://www.fiu.edu/~barbieri Look for……Teaching… GENETICS General course information including the course syllabus as well as class announcements, chapters and additional required readings. It is your responsibility to check the course website regularly for updated information and announcements

14. F0 and IN grades A student who earns a failing grade based on the total number of points and fails to complete at least 60% of the course requirements will receive a grade of F0 (F zero). A grade of IN (Incomplete) will only be given in extremely rare circumstances where unusual circumstances occur after the Drop date. For a student to receive an IN, the student must have completed at least half the course work and have a passing grade at the time of the incident. Professional documentation, including an address and telephone number must demonstrate that the student cannot complete the course due to factors beyond their control (severe illness, accident, death in the immediate family, etc.). The student must complete the course within two terms, otherwise the grade will automatically default to an F.

15. Academic Integrity Collaboration is an important aspect of scientific discovery as well as learning. While it is encouraged that students study together and share data with partners as appropriate, all assignments should be written independently by each student. When appropriate, sources (including the internet and personal communication) should be cited. Cheatingand plagiarism are serious offenses and are NOT acceptable. Plagiarism is the presentation of another’s work or ideas (either published or unpublished) as one’s own. This includes both quoting verbatim as well as paraphrasing ideas without appropriate citation. If found to have cheated or plagiarized, or have enabled someone else to do so, the student will be subjected to disciplinary action that will include receiving a zero that cannot be dropped for the assignment/exam. A second occurrence will result in the student receiving an F for the course. If you are aware of such academic dishonesty, report it to the professor.

16. Behavioral Misconduct We (students and instructors) are responsible for maintaining an appropriate, orderly, learning environment in accordance with the mission of the University. Disruptive behavior will not be tolerated. All cell phones must be turned off. During exams all electronic devices must be turned off and put away. Students in attendance should listen and participate appropriately. Students who fail to adhere to the behavioral expectations outlined by the instructor and presented in the FIU Student Handbook may be subjected to discipline in accordance with the procedures described in the FIU Student Handbook. The student handbook also describes the University's policy on sexual harassment.

17. ADA Accommodation Statement If the student has a documented disability as described by the Rehabilitation Act of 1973 (PL 933-112 Section 504) and Americans with Disabilities Act (ADA) and would like to request academic and/or physical accommodations, please contact the Disability Resource Center (tel. 305-348-3532, TTY/TDD 305-348-3852), as soon as possible. Course requirements will not be waived, but reasonable accommodations be provided as appropriate. It is then the student's responsibility to contact and meet with the instructor as early as possible.

18. Extra credits: class assignments Genetic Disease Project Dr. Barbieri will choose one of the diseases below for each team. It is each person’s responsibility to research and prepare 3-4 pages description of the elected disease You need to be: -Concise -Clear -Work as a team -DO not include pictures, graphs, models, cartoons, etc

19. Genetics Research Project • One member of the group will be responsible for insuring this research project is completed. • It has to be sent (via email) to Dr. Barbieri on TIME as a “PDF” by 6/28/2012 (11:59 pm). • The research project needs to be signed by each member of the team.

20. Genetics Research Project • Selected Genetics Disease • 1-Cystic Fibrosis • 2-Huntington’s Disease • 3-Tay-Sachs disease • 4-Ataxia Telangiectasia • 5-Neurofibromatosis • 6-Down syndrome • 7-Color blindness • 8-Haemophilia • 9-Duchenne muscular dystrophy • 10-Amyotrophic lateral sclerosis (ALS)

21. Genetics Research Project -Please use Arial 11 or Times 12 -Margins: 1”, 1”, 1”, 1” Cover page: Group number: 1 to 10 Name, PID, date and signature Project: --Genetics of the disease (2 pages) --Cell Biology of the disease (3/4 pages) --Pathophysiology of the disease (3/4 pages)

22. Genetics The study of the function, structure and activities of nucleic acids…

23. Soft drinks and PCB3063 SUGARS Na+ Phosphoric acid pH~2.1 Caffeine H20 = CO3H- =CO2= Nature’s herbs extract Source of Energy?

24. SUGARS Intestinal Lumen Blood (?)

25. Pancreas Blood beta cells (sugars) (?)

26. Glucose Regulation of β-pancreatic Cells ? (Blood) Sugars Blood

27. Insulin Receptor-Liver- Blood (Replication) Transcription Translation of genes

28. Control of Gene Expression in Eukaryotes • Genes are turned on and off as a result of interaction with regulatory proteins. • Each cell type contains a unique set of proteins. • Regulation of gene expression occurs on three levels: • Transcriptional/Processing-level control • Translational-level control

29. -Eukaryotes • ---Genomic control • ---Transcriptional control • Transcriptional-level control • Processing-level control • ---Posttranscriptional control

30. Organization of a bacterial operon

31. Cell and Molecular Biology

32. Modern Cell (and Molecular) Biology Cytology the study of cell structure Genetics the study of the behavior of genes Biochemistry the study of the chemistry of living systems

33. Barbieri’s Lab We investigate the role of bio-signaling receptors Interaction between cell surface receptor and hormones Insulin-Receptor ---------Diabetes Growth Factor-Receptor----Breast Cancer Interaction between bacteria- host cells Salmonella- Pseudomonas-Listeria --------Infectious Diseases Are you interesting in Biomedical Research?

34. Phagocytosis of Listeria moncytogenes in macrophages expressing Rab5-GFP