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InvoIvement of Chloroplasts in the Programmed Death of Plant cells

InvoIvement of Chloroplasts in the Programmed Death of Plant cells. ABSTRACT. CN - : apoptosis inducer. Guard cell – chloroplast&mitochondria. Epidermal cell – only mitochondria. Prevented by antioxidant. Anaerobiosis

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InvoIvement of Chloroplasts in the Programmed Death of Plant cells

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  1. InvoIvement of Chloroplasts in the Programmed Death of Plant cells

  2. ABSTRACT

  3. CN- : apoptosis inducer Guard cell – chloroplast&mitochondria Epidermal cell – only mitochondria Prevented by antioxidant Anaerobiosis Protein kinase C inhibitor staurosporine Cystein&serine protease inhibitors Suppressed by electron acceptors Only guard cell but not epidermal cell

  4. Methyl viologen - no influence on the removal of CN‾ -induced apoptosis in guard cells under anaerobic conditions DCMU (3-(3’.4’-dichlorophenyl)-1,1-dimethylurea) - an inhibitor of the electron transfer in PhotosystemII DNP-INT (dinitrophenylester of iodonitrothymol) - an antagonist of plastoquinol at the Q site of the chloroplast cytochrome b6f complex Supressed for CN‾ induced apoptosis of guard cells by DCMU and DNP-INT Apoptosis initiation in guard cells depends on the simultaneous availavility of two factors

  5. INTRODUCTION

  6. Cell death in plant cell

  7. PCD(programmed cell death) in the cereal aleurone PSV : protein storage vacuole

  8. Cell death in plant

  9. PCD mechanisms in plant cell

  10. To investigate the role of chloroplast the involvement of light-dependent process in PCD In plant Apoptosis can be caused by cyanide Not extraneous compounds for plants, synthesized by plants Illumination promoted the CN‾ induced apoptosis in guard cells Guard cell – more CN‾ resistant than epidermal cells Insignificant in the dark in guard cells of apoptosis

  11. Summary of redox activities that control gene expression if higher plant cells

  12. RESULT

  13. Nucleus destruction,% Effects of antioxidants and anaerobiosis in epidermal and guard cells Guard cells Epidermal cells Epidermal, guard cells

  14. Nucleus destruction,% Epidermal cells Effects of MV and menadione Guard cells

  15. Nucleus destruction, % aerobiosis amaerobiosis Effects of MV in guard cell

  16. Effects of BQ,DAD, TMPD, and DPIP Guard cells Guard cells Epidermal cells Nucleus destruction,% light dark dark

  17. Effects of DCMU and DNP-INT Epidermal cell Guard cell

  18. Effects of the inhibitors of protein kinase C and proteases Guard cells Nucleus destruction, %

  19. Conditions required for the CN induced nucleus destruction in guard cells in epidermal films from pea leaves

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