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OOGENESIS OVOGENESIS PADA IKAN

OOGENESIS OVOGENESIS PADA IKAN. OOGENESIS Faktor internal : hormone reproduksi. Faktor eksternal : lingkungan & pakan. Tahap 1 : Sel telur primitive (oogonia/ ovogonium/ archovogonium). Ø : 8 - 12 µ. Mitosis berkali-kali  masuk tahap oosit primer. Tahap 2 :

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OOGENESIS OVOGENESIS PADA IKAN

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  1. OOGENESIS OVOGENESIS PADA IKAN

  2. OOGENESIS • Faktor internal : hormone reproduksi. • Faktor eksternal : lingkungan & pakan. Tahap 1 : • Sel telur primitive (oogonia/ ovogonium/ archovogonium). • Ø : 8 - 12 µ. • Mitosis berkali-kali  masuk tahap oosit primer.

  3. Tahap 2 : • Mulai dikelilingi sel-sel folikel (melindungi & memelihara). • Ø : 12 - 20 µ. Tahap 3 : • Oosit sudah dikelilingi sel-sel folikel. • Ø : 20 - 40 µ. • Awal penimbunan globul-globul yolk (recrudescence).

  4. Tahap 4 : • Awal vitellogenesis • Ø : 200 - 350 µ. Tahap 5 : • Fase II vitellogenesis. • Ø : 350 - 500 µ. • Sitoplasma dipenuhi globul lemak.

  5. Tahap 6 : • Fase II vitellogenesis. • Ø : 600 - 900 µ. • Yolk mendesak lemak ke tepi. • Sintesis protein dalam nucleoli. Tahap 7 : • Akhir vitellogenesis • Ø : 900 – 1.000 µ. • Pembentukan yolk terhenti. • Terbentuk mikrofil. • Fase dormance sampai terjadi ovulasi

  6. Ovulasi : Keluarnya sel telur masak setelah meiosis I dari folikel ke dalam rongga ovarium.

  7. Meiosis I : dalam ovarium  ovulasi • Meiosis II : Setelah pemijahan.

  8. GtH (teleostei) : • Sintesis steroid untuk pematangan dinding folikel. • Sekresi mediator ovulasi. • Target Gth : sel teka folikel membentuk hormone steroid (17α, 20ß dihidroksi-4 pregnen – 3 one / 17, 20 P)  Gold fish (carasius auratus). Hormon tiroid sinergis aktif dengan Gth : • Perkembangan ovarium. • Meningkatkan sensitifitas terhadap GnRH.

  9. 4. stage IV oocyte 5. stage V oocyte 2. stage II oocyte 3. stage III oocyte 1. Connective tissue of thetunica albuginea 6. atretic oocyte Figure 1. Ovary (Formalin, H&E, Bar = 412 µm).

  10. 3. stage IV oocyte 7. ovarian vein 5. early stage V oocyte 6. atretic oocyte 2. stage III oocyte 4. late stage V oocyte 1. connective tissue of thetunica albuginea Figure 2. Ovary (Formalin, H&E, Bar = 186 µm).

  11. 6. vitelline envelope (chorion) Figure 3. Stage III oocytes (Formalin, H&E, Bar = 36.4 µm). 8. yolk granules of stage V oocyte 5. follicular cell layer 7. yolk vacuoles of stageV oocyte 1. nucleus 3. provitelline nucleoli 2. cytoplasm 4. fibrous connective tissue stroma

  12. Figure 4. Stage V vitellogenic oocyte (Formalin, H&E, Bar = 36.6 µm). 5. vitelline envelope(chorion) 2. yolk vacuoles 6. follicular cell layer 1. germinal vesicle 3. yolk granules

  13. Figure 5. Stage V oocytes, detail of the follicular wall (Formalin, H&E,Bar = 21.8 µm). 2. vitelline envelope (chorion) 3. oviplasm 1. follicular cell layer 4. yolk vacuoles 5. yolk granules

  14. Oocyte nucleus

  15. Oocyte cytoplasm

  16. Yolk vacuoles

  17. Less mature oocytes

  18. Macrophages

  19. Sekian dan Selamat Belajar

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