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Supplementary Figure S1. Oh et al. hTERT -(Ub)n. -. -. Hdm2 HA-Ub FLAG-hTERT MG132. +. a. b. -. +. +. +. +. +. +. +. +. -. MG132 Hdm2 HA-Ub FLAG-hTERT. +. -. -. -. -. +. +. -. -. +. +. +. +. 198. +. +. +. +. +. +. IP: HA. 115. 198. hTERT -(Ub)n.

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  1. Supplementary FigureS1. Oh et al. hTERT -(Ub)n - - Hdm2 HA-Ub FLAG-hTERT MG132 + a b - + + + + + + + + - MG132 Hdm2 HA-Ub FLAG-hTERT + - - - - + + - - + + + + 198 + + + + + + IP: HA 115 198 hTERT -(Ub)n 90.5 WB: FLAG IP: FLAG 115 WB: FLAG 90.5 WCE WB: HA WB: Hdm2 1 2 3 WB: FLAG WB: FLAG WCE WB: Hdm2 1 2 3 4 5 6

  2. Supplementary FigureS2. Oh et al. WCE IP: HA + + + + Hdm2 C464A hTERT-HA hTERT -(Ub)n hTERT -(Ub)n - - + + WB: Hdm2 WB: HA 1 2 3 4 a b d  Ring WT - - Hdm2 HA-Ub FLAG-hTERT MG132 C464A WT - - Hdm2 HA-Ub FLAG-hTERT MG132 - + + + - + + + + + + + + + + + + + + + + + + + 198 198 115 IP: FLAG c IP: FLAG 90.5 115 C464A wt WB: HA - Hdm2 C464A hTERT-HA WB: HA 90.5 + + + WB: FLAG WB: FLAG WB: HA WB: FLAG WB: FLAG WB: Hdm2 WCE WCE WB: Hdm2 WB: Hdm2 WB: GFP 1 2 3 4 1 2 3 4 1 2 3

  3. Supplementary FigureS3. Oh et al. + + + + + + + + + + a b 294-491 1-301 WT - - Myc-Hdm2s His-Ub hTERT-HA MG132 - + + + + 294-489 1-301 NC PC wt Myc-Hdm2 - + + + + hTERT hTERT -(Ub)n 198 PD: Ni-NTA 115 WB: HA WB: HA 50 bp WT 115 WCE IC (36bp) 1-301 90.5 294-491 61.5 WB: Myc 1 2 3 4 5

  4. Supplementary FigureS4. Oh et al. a b 350-1132 595-1132 hTERT 1-542 1-350 hTERT-HA Hdm2 - - - - - + + + + + 115 115 90.5 90.5 1 595 946 1132 61.5 61.5 hTERT hTERT RT 46.2 IP: Hdm2 46.2 1-542 37.8 37.8 595-1132 Hdm2 1-350 350-1132 115 115 90.5 90.5 61.5 61.5 46.2 hTERT 46.2 WCE 37.8 37.8 Hdm2

  5. Supplementary FigureS5. Oh et al. - - FLAG-Hdm2 HA-Ub Myc-hTERT MG132 + - + + + + + + + + - FLAG-Hdm2 hTERT-HA GFP + 198 kDa + + hTERT -(Ub)n + + IP: Myc hTERT-HA 115 FLAG-Hdm2 90.5 GFP WB: HA WB: Myc Mock-2 Mock-1 WB: Myc SC-10 SC-7 WCE WB: Hdm2 FLAG-Hdm2 1 2 3 Actin a b c IP: FLAG WCE - - FLAG-Hdm2 hTERT-HA + + + + + + WB: HA WB: FLAG 1 2 3 4 1 2 d e Mock1 Mock2 SC-10 SC-7 NC PC 50 bp 1 2 3 4 IC (36bp) 1 2 3 4 5 6

  6. Supplementary FigureS6. Oh et al. a b c R1 FLAG-negative R1 FLAG-negative R1 HA-negative R2 FLAG-positive R2 FLAG-positive R2 HA-positive

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