1 / 9

Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai

PRODUCTION OF 1,3-PROPANEDIOL THROUGH METABOLIC ENGINEERING. Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai. Properties of 1,3-PD. Molecular weight 76.09 Viscous and colorless liquid Miscible with water Density = 1.0597 g/ cm

dexter
Télécharger la présentation

Advisor: Wei-Shou Hu Speaker: Justice Akhuamhenkhun Ace Lai

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PRODUCTION OF 1,3-PROPANEDIOL THROUGH METABOLIC ENGINEERING • Advisor: Wei-Shou Hu • Speaker: Justice Akhuamhenkhun • Ace Lai

  2. Properties of 1,3-PD • Molecular weight 76.09 • Viscous and colorless liquid • Miscible with water • Density = 1.0597 g/ cm • MP = -28; BP, between 210 to 212 (celsius degree) • Unsaturated compound with hydroxyl functional group

  3. Applications • copolyesters, polymers, textile fibres, and carpets • organic solvent ingredient of wood paints and anti-freeze • chief ingredient manufacturing adhesives, laminates, terracota- as protective reagent

  4. Why we use bioengineering • Cheaper in cost • Easier in designing pathway • Greener to environment

  5. Metabolic pathway

  6. Metabolic engineering I • nature of synthesizing of 1,3PD • tpi gene • dhaT and dhaB, gene encoding glycerol dehydratase and PDOR • DAR1, GPP2 encoding 3-phosphate dehydrogenase and glycerol 3-phosphate phosphatase

  7. Metabolic engineering II • Redesigning of yqhD • Use of NADPH instead NADH • knock down of tpi to manipulate pathway more efficiently

  8. Calculation analysis • Yield, 1,3-propanediol at a rate of 3.5 g/L/h, a titer of 135 g/L, and a weight yield of 51% in D-glucosefed-batch 10 L fermentations.

  9. Conclusion • DAR1 and GPP2 cloned from Saccharamyces cerevisiae encoding 3-phosphate dehydrogenase • dhaB, dhaT gene from Klebsiella pneumoniae encoding PDOR and glycerol dehydratase • yqhD from E. coli can produce PDOR as well • tpi manipulated via knockdown to resolve carbon flux • PEP and ATP dependent glucose pathway modified • In all, contribute to higher yield synthesis

More Related