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UD. IV. GENÈTICA. Ll. IV. 5. Biotecnologia

UD. IV. GENÈTICA. Ll. IV. 5. Biotecnologia 4. Estudi de la seqüència d’ADN . Mètode didesoxi. TECHNIQUE. Figure 20.12. Primer. Deoxyribonucleotides. Dideoxyribonucleotides (fluorescently tagged). DNA (template strand). 3 . T. G. 5 . C. T. dATP. ddATP. T. T. 5 . G. dCTP.

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UD. IV. GENÈTICA. Ll. IV. 5. Biotecnologia

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  1. UD. IV. GENÈTICA. Ll. IV. 5. Biotecnologia 4. Estudi de la seqüènciad’ADN. Mètodedidesoxi.

  2. TECHNIQUE Figure 20.12 Primer Deoxyribonucleotides Dideoxyribonucleotides(fluorescently tagged) DNA(template strand) 3 T G 5 C T dATP ddATP T T 5 G dCTP ddCTP A DNApolymerase dTTP C ddTTP T dGTP T ddGTP C G P P P P P P A G G C A OH H 3 A DNA (templatestrand) Labeled strands 3 5 ddG C A T ddA C G ddC C T A T ddT T G C ddG G G G A T A A A A ddA A ddA T A A A A A G G C ddG G G G G G C C G C ddC C C C C C T T T T T A T T T T T T T T T T T T C G G G G G G G G G T T A T T T T T T T 5 T T 3 T T T T A T T T Shortest Longest Directionof movementof strands Longest labeled strand Detector Laser Shortest labeled strand RESULTS Last nucleotideof longestlabeled strand G A C T G A Last nucleotideof shortestlabeled strand A G C

  3. Figure 20.12a TECHNIQUE Deoxyribonucleotides Primer Dideoxyribonucleotides(fluorescently tagged) DNA(template strand) 3 T G 5 C T dATP ddATP T T 5 G ddCTP dCTP A DNApolymerase dTTP C ddTTP T dGTP ddGTP T C G P P P P P P A G G C A H OH A 3

  4. didesoxinucleòtid

  5. Figure 20.12b TECHNIQUE (continued) DNA (templatestrand) Labeled strands 3 5 ddG C A T ddA C G ddC C T A T ddT T G C G G ddG G A A A A T A ddA ddA A A A A T A A G G G G C ddG G G G C G C C C C C ddC C C T T T A T T T T T T G G G C G G G G G G T T T A T T T T T T T T T T A T T T T T 5 3 Shortest Longest Directionof movementof strands Longest labeled strand Detector Laser Shortest labeled strand

  6. Figure 20.12c Directionof movementof strands Longest labeled strand Detector Laser Shortest labeled strand RESULTS Last nucleotideof longestlabeled strand G A C T G A Last nucleotideof shortestlabeled strand A G C

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