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Cardiac Morphology, Gene Expression and Histology (Core D)

Cardiac Morphology, Gene Expression and Histology (Core D). Description of Core Unit: Generate staged mouse and Xenopus embryo RNA samples. Generate paraffin and OCT embedded tissues and embryos. Share expertise in and perform some whole mount and section in situ hybridizations.

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Cardiac Morphology, Gene Expression and Histology (Core D)

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  1. Cardiac Morphology, Gene Expression and Histology (Core D) • Description of Core Unit: • Generate staged mouse and Xenopus embryo RNA samples. • Generate paraffin and OCT embedded tissues and embryos. • Share expertise in and perform some whole mount and section in situ hybridizations. • Perform IHC and histology on tissue and embryo sections.

  2. Core D Progress Report I • Successful use of RT-PCR for Xenopus Mo2L (Carrie Dasconio). • Performing in situ hybridizations (ISH; mouse and Xenopus; Projects 4 & 5, Ning Dai and Carrie Dasconio) including Mo2L and mouse Ehmt1 (Project 4).

  3. Core D Progress Report II • 301 slides stained (1008 total). • 743 slides unprocessed (2186 total). • 15 embedded tissue blocks (141 total). Ning Dai

  4. Core D Prospective • Interactions with other projects are welcome.

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