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Adventures in HRP

Adventures in HRP. Ellyn Daugherty San Mateo Biotech Career Pathway www.SMBiotech.com AEEDaugher@aol.com www.BiotechEd.com EMC/Paradigm Publishing www.emcp.com/biotech VWR/Sargent-Welch www.sargentwelch.com. San Mateo Biotechnology Career Pathway www.SMBiotech.com.

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Adventures in HRP

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  1. Adventures in HRP Ellyn Daugherty San Mateo Biotech Career Pathway www.SMBiotech.com AEEDaugher@aol.com www.BiotechEd.com EMC/Paradigm Publishing www.emcp.com/biotech VWR/Sargent-Welch www.sargentwelch.com

  2. San Mateo Biotechnology Career Pathwaywww.SMBiotech.com Giving motivated teenagers and adults the skills to pursue several career and academic options.

  3. Partnerships with Industry and Academia

  4. Biotechnology is an opportunity to teach science in a way that results in science literacy & research skills & career awareness for virtually every student. • Students learn the skills and gain the confidence and self-directedness that are necessary for independent research(science fair projects) and higher-level science courses. • Students see meaning and application in their math, biology and chemistry. • Students learn the science and business of biotech so that they are better prepared to make decisions about their futures and society.

  5. The HRP Activities are found in Chapter 6 • Text - Chapter 6 and 14 • Lab Manual - Labs 6f-6h, 14a • Student CD Tutorials for basic skills • Course Planner/Instructor’s Guide

  6. Biotech 3 HRP labs: • Peroxidase Assay • HRP extraction from HR Root • HRP assay using PAGE • HRP assay w/ TMB indicator • HRP used in ELISAs

  7. How Do You Make the Phosphate Buffer? You need 100 mL of a 25 mM NaH2PO4 * H20, pH 7 buffer. conc. desired X volume desired X molecular weight = amount of solute in mol/L in L in g/mol in g Convert 25 mM = 0.025 mM and convert 100 mL = 0.1 L 0.025 mol/L X 0.1 L X 138 g/mol = 0.345 g NaH2PO4 * H20 0.345 g of NaH2PO4 * H20 is measured measured on a balance and put into an appropriate vessel. Then, solvent is added up to the 100 mL graduation. 100 mL Adjust pH to 7 and add dH20 to 100 mL add solvent (dH20) to 75 mL 0.345 g NaH2PO4 * H20

  8. HRP is 43 kD 50 kD 35 kD

  9. My Professional Goals Biotechnology • Biotechnology programs in everyhigh school, CTE center, and community college, tailored to the student population and industry and community needs • Aligned and articulated programs in HS and CC so that teenagers and adults have the same platform from which to enter specialized programs • To make it as easy as possible for educators to do the other 2

  10. www.BiotechEd.com

  11. www.BiotechEd.com

  12. Get help starting or improving a biotech program ! Ellyn Daugherty SM Biotech Career Pathway www.SMBiotech.com www.BiotechEd.com www.emcp.com/biotech www.sargentwelch.com/biotech AEEDaugher@aol.com JEFF GANDY 800.848.9033 jgandy@emcp.com Holly Ahern 512-789-3155 holly_ahern @vwreducation.com Sandy Duffey for preview copy sduffey@emcp.com

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