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Using Your Staining Antibodies with BD CompBeads

Using Your Staining Antibodies with BD CompBeads. Verify Ab has Kappa light chain. Match Ab to species. Mouse - use BD#552843. Rat - use BD#552844. Rat/Hamster - use BD#552845. Single stain beads as if 1x10 6 cells. Use amount to match or exceed brightness of stained cells.

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Using Your Staining Antibodies with BD CompBeads

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  1. Using Your Staining Antibodies with BD CompBeads Verify Ab has Kappa light chain Match Ab to species Mouse - use BD#552843 Rat - use BD#552844 Rat/Hamster - use BD#552845 Single stain beads as if 1x106 cells Use amount to match or exceed brightness of stained cells Run as compensation sample Use validated instrument settings Fit beads on scatter plot Gate on singlet beads Acquire sufficient events Flow Core= 15,000 all M. Roederer= 50,000 gated Calculate compensation 060320 / EVC-FCC / Suzanne Mertens / saguila@emory.edu

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