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TRANSGENIC RESEARCH The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers Katja Kempe, Myroslava Rubtsova, David Riewe and Mario Gils

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  1. TRANSGENIC RESEARCH The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers Katja Kempe, Myroslava Rubtsova, David Riewe and Mario Gils LeibnizInstitute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstraße 3, 06466 Stadt Seeland OT Gatersleben, Germany gils@ipk-gatersleben.de - Supplemental Figure 1 - Wt Wt pHW501 pHW681 pHW781 pHW461 pHW491 (a) Pubi HPT II Tnos HW501 Ptap Bar-N IntN Tocs Tocs Bar-C IntC Ptap PSK7-i3 [+108] PSK7-i3 [+108] PSK7-i1 [+108] UBQ10-i1 UBQ10-i1 Ptap Ptap Bar-N Bar-N IntN IntN Tocs Tocs Tocs Tocs Bar-C Bar-C IntC IntC Ptap Ptap HW491 HW461 (GGGGS)3 (GGGGS)3 (GGGGS)3 (GGGGS)3 UBQ10-i1 [UTR] UBQ10-i1 [UTR] UBQ10-i1 HW681 Ptap Bar-N IntN Tocs Tocs Bar-C IntC Ptap (GGGGS)3 (b) PSK7-i3 [+108] UBQ10-i1 [UTR] UBQ10-i1 HW781 Ptap Bar-N IntN Tocs Tocs Bar-C IntC Ptap (GGGGS)3 (GGGGS)3

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