1 / 18

Young Cell 的 分佈

Young Cell 的 分佈. Aty-Lym. Fluorescence (RNA ,DNA). ProMyelo. Myelo. Blast. Meta. Band. Resistant RBC 既沒有 RNA, 也沒有顆粒 , 因此即使有 紅血球破壞不全的現象 , 也不會干擾 WBC 的分類. RBC Ghost. Side Scatter ( 內容物結構 ). Estimation of cell viability by flow cytometry. (Death cells). (Live cells).

fagan
Télécharger la présentation

Young Cell 的 分佈

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Young Cell 的 分佈 Aty-Lym Fluorescence (RNA ,DNA) ProMyelo Myelo Blast Meta Band Resistant RBC既沒有RNA, 也沒有顆粒, 因此即使有 紅血球破壞不全的現象, 也不會干擾WBC的分類 RBC Ghost Side Scatter (內容物結構)

  2. Estimation of cell viability by flow cytometry

  3. (Death cells) (Live cells) Protocol for viability 細胞培養之細胞數約1×105~2×105/well，加入不同濃度藥物，培養不同的時間 ↓ 收細胞 ↓ 加入螢光染劑（PI for viability） ↓ 流式細胞儀分析

  4. Cell cycle analysis

  5. Protocol for cell cycle 細胞培養之細胞數約1×105~2×105/well，加入不同濃度藥物，培養不同的時間 ↓ 收細胞 ↓ 加入70% ethanol，固定細胞。放入-20度冰箱儲存，至少24小時。 ↓ 將-20度中的sample從冰箱取出 ↓ 離心1500rpm，5mins ↓ 倒掉上清液，並打散細胞 ↓ PBS wash ↓ 1500 rpm，5mins ↓ 加入PI（for cell cycle）作用30分鐘 ↓ 流式細胞儀分析 ☆ cell cycle所用的PI與viability所用的PI成分不同

  6. Apoptosis G0/G1 G2/M S

  7. G2/M G2/M G2/M G2/M

  8. G0/G1 G0/G1 G0/G1

  9. Analysis of apoptosis by flow cytometry

  10. Phosphatidylserine (PS) and apoptosis

  11. Apoptosis rate analysis using Annexin V/propidium iodide flow cytometry in cancer cells treated with drug Later stages of apoptosis and necrosis Viable cells Early stage of apoptosis

  12. Apoptotic cells

  13. Detection of intracellular/intranuclear antigens

  14. Detection of intracellular/intranuclear antigens

  15. Tumor Antigen Markers

More Related