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Growing Viruses: Methods and Identification Techniques

Learn about the various methods of growing viruses, including using living cells, animals, and embryonated eggs. Discover how to identify viruses through cytopathic effects, serology tests, and nucleic acid studies.

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Growing Viruses: Methods and Identification Techniques

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  1. Growing Viruses 7 • Viruses must be grown in living cells • Bacteriophages form plaques on a lawn of bacteria Figure 13.6

  2. Growing Viruses 8 • Animal viruses may be grown in living animals or in embryonated eggs Figure 13.7

  3. Virus Identification 9 • Cytopathic effects: Detectable changes in the host cell due to viral infection (p. 441) • Blood serum (serology) tests • Detect antibodies against viruses in a patient • Use antibodies to identify viruses in neutralization tests, viral hemagglutination, and Western blot • Nucleic acids: Studies of the DNA/RNA

  4. Virus Identification: Cytopathic Effect 10 Figure 13.9

  5. Attachment, Entry, Uncoating 14 • By pinocytosis Figure 13.14a

  6. Attachment, Entry, Uncoating 15 • By fusion Figure 13.14b

  7. Multiplication of DNA Virus 16 Figure 13.15

  8. Sense Strand (+ Strand) RNA Virus 17 Figure 13.17a

  9. Antisense Strand (– Strand) RNA Virus 18 Figure 13.17b

  10. Double-Stranded RNA Virus 19 Figure 13.17c

  11. 22

  12. Avian Influenza Clinical Focus, p. 371

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