1 / 16

بسم الله الرحمن الرحيم

بسم الله الرحمن الرحيم. GENUS: CORYNEBACTERIUM Prof. Khalifa Sifaw Ghenghesh. Diptheroides = Coryneforms Gram +ve, Nonmotile Rods. Arranged in V forms or Palisades Catalase +ve. 1. Corynebacterium diphtheriae. Diphtheria. Pleomorphic Bacilli Cells Contain Metachromatic Granules.

Télécharger la présentation

بسم الله الرحمن الرحيم

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. بسم الله الرحمن الرحيم GENUS: CORYNEBACTERIUM Prof. Khalifa Sifaw Ghenghesh

  2. Diptheroides = Coryneforms • Gram +ve, • Nonmotile Rods. • Arranged in V forms or Palisades • Catalase +ve

  3. 1. Corynebacterium diphtheriae • Diphtheria. • Pleomorphic Bacilli • Cells Contain Metachromatic Granules. Morphology

  4. Corynebacterium diphtheriaeClub-shaped, Gram-positive rods

  5. Cultural Characteristics • i. Loeffler's Serum Slope. • ii. Tellurite BA. - Temp. 37oC, - Aerobic and Facultative AnO2.

  6. Corynebacterium diphtheriae forms black colonies on tellurit agar (left), on blood agar colonies appear white (right)

  7. PATHOGENICITY • Diphtheria is a Toxaemia. • Exotoxin: M. wt 62000 • 2 Fragaments >> A & B • Inhibits Protein Synthesis. • Has Special Affinity for Certain Tissues "Mycocardium, Adrenals, Nerve Endings". • Toxin >>Toxoid. • Site of Infection >>Faucial. • Toxin >>Pseudomemebrane.

  8. LABORATORY DIAGNOSIS • Important>>Specific Treatment Should Be Started Immediately on Suspicion of Diphtheria. • Specimen:Two Swabs from the Lesion. • Culture: i. Loeffler's Serum Slope >> Albert Stain. ii. Tellurite BA. iii. BA>> Streptococcal or Staphylococcal Pharyngitis.

  9. PATHOGENICITY TESTS • In-vivo Tests: i. Subcutaneous Test: ii. Intracutaneuos Test: • In-vitro Test: • Elek Test ( Gel Precipitaion Test). • Tissue Culture:

  10. TREATMENT • i. Antitoxin:> Moderate Cases: > Severe Cases: • ii. Antibiotic: • i. Active Immunization >> PDT. • ii. Passive Immunization: • iii. Combined: PREVENTION

  11. SCHICK TEST • > Injection of 0.2 ml of Diphtheria Toxin Intradermally. • > Positive Reaction >> Erythema + Swelling

  12. SCHICK TEST ______________________________________________________________________ Result Test Arm Control Arm Interpretation Diphthri (Toxin) Inactivated Toxin Immunization 36h 120h 36h 120h ______________________________________________________________________ Negative -- -- -- -- Immune, Not Not Hypersensitive Required ---------------------------------------------------------------------------------------- Positive + + -- -- Not Immune, Not Required Hypersensitive --------------------------------------------------------------------------------------------------------------------- Negative + -- + -- Immune Not Hypersensitive Required --------------------------------------------------------------------------------------------------------------------- Positive& + + + -- Not ImmuneContra- PseudoHypersensitive indicated ______________________________________________________________________

  13. بسم الله الرحمن الرحيم Listeria monocytogenes

  14. Listerosis. • G+ve Coccobacilli >in Short Chains. • Motile at 25oC But Not at 37oC. • Aerobic to Microaerophilic. • On BA>Narrow Zone of b-Haemolysis. • Listerolysin O >

  15. DISEASE • Incidence and Transmission > • i. In Adults: • ii. Neonatal Infections: a. Early Type: b. Late Type:

  16. LABORATORY DIAGNOSIS • i. Specimen:Depend on Syndrome: Blood, CSF, Genital Tract Secretions. • ii. Media:Selective Media. • Penicillin, Ampicillin. TREATMENT

More Related