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Gene annotation: Nitrogen transporter families and N metabolism

Gene annotation: Nitrogen transporter families and N metabolism. Annick Brun , Eva Lucic, Marc Buée, Barbara Montanini, Damien Blaudez , Francis Martin et Michel Chalot. Unité Mixte de Recherche 1136 INRA/Université Henri Poincaré Interactions/Arbres Micro-Organismes

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Gene annotation: Nitrogen transporter families and N metabolism

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  1. Gene annotation: Nitrogen transporter families and N metabolism Annick Brun, Eva Lucic, Marc Buée, Barbara Montanini, Damien Blaudez , Francis Martin et Michel Chalot. Unité Mixte de Recherche 1136 INRA/Université Henri Poincaré Interactions/Arbres Micro-Organismes IFR "Génomique, Ecophysiologie et Ecologie fonctionnelle" BP 239 - 54506 Vandoeuvre-lès-Nancy Cedex, France INRA-Nancy, 5 avril 2006

  2. Marmeisse, R., et al. (2005) New Phytologist 163 (3), 481-498.

  3. Laccaria bicolor proteases Approximately 130 proteases in L. bicolor Caspase: involved in apoptosis Fungalysin: membrane attachments, remodeling of cytoskeletal, apoptosis Calpain: probably contributes to destruction of host tissues in infection (plant and animal infections)

  4. Ascomycota cluster(Trichoderma, Aspergillus oryzae, A. nidulans) Basidiomycota (Hymenomycetes) cluster 1(Coprinus, Laccaria, Phanerochaete, Coccidioides) Basidiomycota cluster 2 (Coprinus, Laccaria, Phanerochaete, Ustilago) Potentially involved in the degradation of the OM Basidiomycota cluster 3(Coprinus, Laccaria, Phanerochaete, Ustilago)

  5. OPT

  6. Phanerochaete chrysosporium Coprinus cinereus Tetra / pentapeptide and glutathione transport Laccaria bicolor Cryptococcus neoformans Ustilago maydis

  7. Marmeisse, R., et al. (2005) New Phytologist 163 (3), 481-498.

  8. Yeast Amino Acid Transporter (YAT) Amino Acid/Choline Transporter (ACT) L-type Amino Acid Transporter (LAT) Amino Acid/Auxin Permease (AAAP)

  9. 10 gene models

  10. A AMT1 AMT2.1 AMT3.1 AMT13.2 AMT13.4 B T0 -N48 +N24 Carpo AMT expression by RT-PCR Amino acids content Glutamine content

  11. x2 - x4

  12. KEGG Metabolic Pathways Energy Metabolism 130 ATP synthesis 17       Carbon fixation 19       Methane metabolism 3       Nitrogen metabolism 32       Oxidative phosphorylation 37       Photosynthesis 1       Reductive carboxylate cycle (CO2 fixation) 14       Sulfur m etabolism 7 Amino Acid Metabolism 494       Alanine and aspartate metabolism 25       Arginine and proline metabolism 34       Cysteine metabolism 9       Glutamate metabolism 32       Glycine, serine and threonine metabolism 46       Histidine metabolism 30       Lysine biosynthesis 22       Lysine degradation 46       Methionine metabolism 17       Phenylalanine metabolism 24       Phenylalanine, tyrosine and tryptophan biosynthesis 18       Tryptophan metabolism 92       Tyrosine metabolism 36       Urea cycle and metabolism of amino groups 20       Valine, leucine and isoleucine biosynthesis 12       Valine, leucine and isoleucine degradation 31

  13. Carbonate dehydratase 9,3,0 Carbamoyl-phosphate synthase1,0,1 Aminomethyltransferase 2,1,1 Phenylalanine ammonia-lyase 2,1,2 D-amino-acid dehydrogenase1,2,1 Asparaginase 3,0,1 GDH à NAD 2,1,1 Glutamate synthase NADPH 1,1,1 GDH à NADPH 1,1,1 Alanine Amino Transférase (1,1,1) Asparagine synthase 1,1,1 GS (1,1,1) Aspartate Amino Transférase (2,3,3) Cystathionine gamma-lyase 4,1,5 Laccaria laccata Phanerochaete chrysosporium Cryptococcus neoformans 2-nitropropane dioxygenase (1,1,1) Nitrite reductase1,1,1 Nitrate reductase1,1,1

  14. Argininosuccinate synthase (2,1,1) Argininosuccinate lyase (2,1,1) Ornithine carbamoyle transferase (2,1,1) Arginase (1,1,1) Aminoacylase(1,1) Carbamoyl-phosphate synthase (1,1,1) Glutamate N-acetyltranferase (1,1,1) Acetylornithine aminotransferase (2,2,2) Guanidinoacetate N-methyltransferase (1,1,1) Protein ARG5,6(1,1) Urease (1,1,1) Ornithine-oxo-acid aminotransferase (1,1) Ornithine decarboxyase (1,1,1) Amino-acid N-acetyltransferase (2,2,2) Glutamate-5-semialdehyde dehydrogenase (1,1,1) Glutamate 5-kinase (1,1,1) Pyrroline-5-carboxylate reductase (1,1) Cycle de l’urée Laccaria laccata Phanerochaete chrysosporium Cryptococcus neoformans 1 1 Spermidine Synthase(1,1,1) 2 Chimeric Spermidine Synthase/ Saccharopine Dehydrogenase(1,2,1) 2

  15. Read, D. J. & Perez-Moreno, J.(2003) New Phytologist 157 (3), 475-492.

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