1 / 12

Spectrophotometer

Spectrophotometer. An instrument for measuring the intensity of light of a definite wavelength transmitted by a substance or a solution. Visible Spectrophotometer U.V Spectrophotometer. Electromagnetic spectrum.

garret
Télécharger la présentation

Spectrophotometer

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Spectrophotometer

  2. An instrument for measuring the intensity of light of a definite wavelength transmitted by a substance or a solution. Visible Spectrophotometer U.V Spectrophotometer

  3. Electromagnetic spectrum

  4. A spectrophotometer measures how light interacts with atoms or molecules in a sample.

  5. Components of spectrophotometer A spectrophotometer consists of a light source , a prism that separates the light into different wavelengths, a slit through which a narrow beam of the desired wavelength passes, a sample holder, a detector , and a recording device

  6. Effect of concentration on absorbance

  7. Beer Law • Beer Law states that there is a linear relationship between the absorbance and the concentration of a sample. A = = L [C] More dissolved substance = more absorption (A) and less transmittance (T)

More Related