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Population genetics in different contexts: Impacts of altitude & landscape structure. Thomas Hahn Thomas.hahn@env.ethz.ch. BioChange. Population genetic factors. Size Isolation Age Mating system Selection. 1/8. Questions I (altitude).
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Population genetics in different contexts: Impacts of altitude & landscape structure Thomas Hahn Thomas.hahn@env.ethz.ch BioChange
Population genetic factors • Size • Isolation • Age • Mating system • Selection 1/8
Questions I (altitude) Are upper peripheral populations compared with central ones : 0) more isolated & smaller? 1) genetically depauperate? 2) genetically more differentiated? ? ? 2/8
? Questions I (pollination) • Population genetic differences among insect- and wind-pollinated species? 3/8
Methods I n = 30 1800 m 1200 m 10 x AFLP-Analyses 4/8
Questions II (altitude & landscape) • How are metapopulations structured along an altitudinal gradient? • What are directions, corridors & barriers for gene flow in metapopulations? From Holderegger & Wagner, 2008 (changed) 5/8
Methods II ¦ 1000 m ¦ 3 x 15 Subpopulations à 30 Individuals GIS 6/8
A further question • Differences in clonal reproduction among central and upper peripheral populations in B. media? 7/8
From Murray, 1976 Challenges • Two cytotypes in B. media in Switzerland! • Distribution of cytotypes within & among populations, regions or heights? • Gene flow among cytotypes? Flow cytometry analyses: 8/8