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Comparative Proteogenomics: Lessons learned from the excercise

Comparative Proteogenomics: Lessons learned from the excercise. Samuel H Payne J Craig Venter Institute. Proteogenomics Protocol. Practice Makes Perfect. 30 proteomes in 3 years. Future. Comparative Proteogenomics What can be learned with multiple datasets?. Practice Reveals Flaws.

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Comparative Proteogenomics: Lessons learned from the excercise

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  1. Comparative Proteogenomics:Lessons learned from the excercise Samuel H Payne J Craig Venter Institute

  2. Proteogenomics Protocol

  3. Practice Makes Perfect • 30 proteomes in 3 years

  4. Future • Comparative Proteogenomics • What can be learned with multiple datasets?

  5. Practice Reveals Flaws • Conflict resolution

  6. ORF Filtering • Peptide uniqueness • Min peptide/protein • Length clustering (GC rich genomes) • Tryptic content of peptide set • Sequence complexity (GA rich peptides)

  7. Acknowledgements • Eli Venter • Dick Smith, PNNL • NSF

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