1 / 17

PEMURNIAN DAN KARAKTERISASI ENZIM ASETIL-COA HIDROLASE

PEMURNIAN DAN KARAKTERISASI ENZIM ASETIL-COA HIDROLASE. Donny Nugraha M 20508024. PENDAHULUAN. MEKANISME REAKSI. AGENDA. TEKNIK ANALISIS. PENGGUNAAN. KESIMPULAN. PENDAHULUAN. Senyawa yang dioksidasi siklus Krebs. ..?. ASETIL- CoA.

gunda
Télécharger la présentation

PEMURNIAN DAN KARAKTERISASI ENZIM ASETIL-COA HIDROLASE

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PEMURNIAN DAN KARAKTERISASI ENZIM ASETIL-COA HIDROLASE Donny Nugraha M 20508024

  2. PENDAHULUAN • MEKANISME REAKSI AGENDA • TEKNIK ANALISIS • PENGGUNAAN • KESIMPULAN

  3. PENDAHULUAN Senyawa yang dioksidasisiklus Krebs ..? ASETIL-CoA “As donor substrats in co-translational, post-translational or chemical modification : acetylation”

  4. PENDAHULUAN • NC-IUBMB3.1.2.1 • Acetyl-CoAacylase, acetyl-CoAthiol esterase, acetyl-CoAdeacylase, Ach1 • Pertamaditemukandihatibabi (1952) • Hati & otakTikus, Jaringanadiposa Hamster, Saccharomyces cerevisiae

  5. MEKANISMEREAKSI MengkatalisisreaksihidrolisisAsetil-CoAmenjadiAsetatdanCoA

  6. TEKNIKANALISIS Gel Filtration chromatography DEAE – Sepharose SDS-PAGE (Sodium DodecylSulphate- PolyAcrilamide-Gel Electrophoresis)

  7. TEKNIKANALISIS Homogenity and Molecular Properties Menggunakan prosedur kromatografi DEAE-Sepharose, Sepharose CL-4B, Hidroxilapatit Enzim terdiri kira-kira 0,2 % dari total “Cellular protein” Molecular Weight Analysis Menggunakan elektroforesis jel - metode Laemmli dan dibandingkan dengan berat molekul standar (miosin 205kDa, Bovin serum albumin 66kDa) Mr 64,000 ± 5,000 . monomeric

  8. TEKNIKANALISIS Isoelectric Analysis Pita “ Single protein” padapI=5,8 pH Dependence Analysis Pengujian pH dari 4 Sampai 10

  9. TEKNIKANALISIS Temperature Optimum Analysis Aktivitasenzimdari 5 sampai 65C ,maksimumterjadipada 37 -42C Amino Acid Analysis

  10. TEKNIKANALISIS Substrat Specificity Analysis

  11. PENGGUNAAN Pharmaceutical Uses Enzyme would inhibit acetylation of platelets, thus increasing clotting time Agriculture Uses

  12. KESIMPULAN • Mr 64,000 ± 5000 Da • Isoelectric point 5,8 • pH optimum 8,0 • Temperatur Optimum 37 – 42C • Irreversibeldenaturasisetelah 1 menitpada 70C • Substrattergantungspesifisitas

  13. SEKIAN & TERIMAKASIH

More Related