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Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes. GFP-RAS2 in living Saccharomyces cerevisiae. Images from Roger Tsein’s Lab. Making a Fusion Protein:. Yak1 Gene: DNA: AAAG ATG AATCAG ... ACATCG TGA CCTA... Protein: N- Met-Asn-Gln-…-Thr-Ser-C

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Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

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  1. Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

  2. GFP-RAS2 in living Saccharomyces cerevisiae

  3. Images from Roger Tsein’s Lab

  4. Making a Fusion Protein: Yak1 Gene: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C GFP Gene: DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... Protein: N-Met-Ser-Lys-… -Leu-Lys-C Yak1 GFP

  5. Making a Fusion Protein: • Yak1 Gene: • DNA: AAAGATGAATCAG ... ACATCGTGACCTA... • GFP Gene: • DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... • Fusion Gene: • AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

  6. Making a Fusion Protein: Yak1 Gene: DNA: AAAGATGAATCAG ... ACATCGTGACCTA... Protein: N-Met-Asn-Gln-…-Thr-Ser-C GFP Gene: DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC... Protein: N-Met-Ser-Lys-… -Leu-Lys-C Yak1 GFP N C Fusion Gene: AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA... Fusion Protein: N-Met-Asn-Gln-…-Thr-Ser-Met-Ser-Lys-… -Leu-Lys-C

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