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Specificity of Antibodies: A Simulation

Specificity of Antibodies: A Simulation. The Patient: Figaro. Antibody Review. What type of macromolecule is an antibody? What shape is a typical antibody?. Protein. Antibody Review. What is an antigen? How many antigens can the antibody below bind to?. Foreign Molecule.

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Specificity of Antibodies: A Simulation

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  1. Specificity of Antibodies: A Simulation The Patient: Figaro

  2. Antibody Review • What type of macromolecule is an antibody? • What shape is a typical antibody? Protein

  3. Antibody Review • What is an antigen? • How many antigens can the antibody below bind to? Foreign Molecule

  4. A day in Figaro's Life

  5. Figaro Possible Antigens

  6. Ways to Test for Allergies Brain storm ways to test for possible allergies.

  7. The Antibody Simulation • Label 3 Petri Plates with initials, date, period, LB Agar, and TRIAL 1, 2 or 3 (depending on the plate).

  8. The Antibody Simulation • Cut the bottom off a large transfer pipet.

  9. The Antibody Simulation • Apply a slight suction by compressing the bulb of the pipet between your fingers.

  10. The Antibody Simulation • Use the transfer pipet to poke holes in the agar. • Make 4 holes around the edge of the agar.

  11. The Antibody Simulation • Make 1 hole in the center of the agar. 1 2 C 3 4

  12. Allergen 1: ? Allergen 2: ? Allergen 3: ? Allergen 4: ? The Antibody Simulation • In your notebook, draw a Petri plate and label which antigen goes into each outside hole. 1 2 3 4

  13. Figaro’s Blood Serum: Antibodies The Antibody Simulation • On your Petri plate drawing in your notebook, label the center hole as Figaro’s Blood Serum. C

  14. The Antibody Simulation • Use a sterile 1 mL pipet to add antigen/ antibody solution to each hole in the agar.

  15. The Antibody Simulation • Add enough antigen serum so the solution is at the top of the agar hole.

  16. The Antibody Simulation • Use a different sterile 1 mL pipet for each antigen solution.

  17. The Antibody Simulation • Fill the center hole with Figaro’s Blood Serum (antibody solution).

  18. The Antibody Simulation • Repeat these steps for all 3 trials/ Petri plates. • Cover all 3 Petri plates and leave undisturbed for 24 hours.

  19. Data Collection • Collect Petri plates. • Draw Petri plate results in your notebook. • Write down observations • Form a data table in Excel. • Create numeric scale to convert observations to data. • Collect data from each trial. • Average the data from trials.

  20. Precipitin Lines The Antibody Simulation: Results • A precipitin line will appear between one or more of the allergens and the blood serum.

  21. The Antibody Simulation: Results • Record results of each of the 3 trials using a numeric scale • 5 = strong precipitation; 0 = no precipitation • Calculate average results for each antigen

  22. Weak Allergy Strong Allergy The Antibody Simulation: Results • The brighter precipitin lines, the stronger the allergy.

  23. Agglutination = Precipitin Lines The Antibody Simulation: Results • This lab is an example of an Ouchterlony test. • When antibodies diffuse in agar and bind with antigens, it causes agglutination, or a white precipitin line.

  24. The Antibody Simulation: Results • Make an individual data table in Google documents. • On the Smart Board, add your group’s data to the table and make a class average data table on your Google spreadsheet below your individual data table.

  25. Antibody Simulation: Conclusion • Post your individual data table, class average data table, and your REE PE PA three-paragraph conclusion on a new page for this lab on your Google Site. • Results - Go back to the purposes of the lab. • Evidence - Numerical evidence. • Explanation - Go back to your hypothesis. • Possible Errors • 2 examples of errors • Avoid future errors • Practical Applications • Suggestions to patient/family. • Further testing • Value of this lab/test

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