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LIFE 12 ENV IT 000652 Cleansed

Optical and Atomic Force Microscopy for analysis of wet sediments from the Canale dei Navicelli. LIFE 12 ENV IT 000652 Cleansed. Analysis with Optical Microscopy (shape, size, granulometry) Analysis with scanning probe by AFM (Atomic Force Microscope). Two methods.

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LIFE 12 ENV IT 000652 Cleansed

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  1. Optical and Atomic Force Microscopy for analysis of wetsedimentsfrom the Canale dei Navicelli LIFE 12 ENV IT 000652 Cleansed

  2. Analysis with Optical Microscopy (shape, size, granulometry) • Analysis with scanning probe by AFM (Atomic Force Microscope) Twomethods

  3. Biologicalmicroscope with high-resolution(16 Mpixel) reflex on trinocular tube for digitalimaging Software: ImageJ(developed by the National Institute of Health): for quantitative analysis (realdimensions-pixel, particlecounting) HeliconFocus Pro: for image stacking (to increase the depth of field) Optical Microscopy

  4. NanosurfEasyscan 2 Microscope • Scanning head: 110*110 micron • Maximum Z range: 22 micron • Maximum resolution: 2048*2048 pixel AFM Microscopy

  5. The texture has great importance for the physical and biological properties of the soil • Classification (USDA): • clay, diameter less than 2 micron • silt, diameter between 2 and 50 micron • sand, between 50 micron and 2 mm. Granulometry

  6. Sample 1 Total counting: 4002 Sample 3 Total counting: 2005

  7. Sample 5 Total counting: 2013 Sample 7 Total counting: 3442

  8. Sample 1 Cyclotella Meneghiniana (MC) Cyclotella Meneghiniana (Moder. Cont.) Staurastrum anatinum (MC)

  9. Sample 1 Cyclotella Meneghiniana (MC) Melosira Varians (MC) Melosira Moniliformis (MC) Navicula Rhyncotella (MC)

  10. Sample 1 Cyclotella Meneghiniana (MC) Melosira Moniliformis (MC) Cyclotella Meneghiniana (MC) Bacillaria Paxillifer (MC to HC)

  11. Sample 1 AFM measurements of sedimentary rock composed by centric

  12. Sample 1 AFM: trace of diatom (Nitzschia Acicularis (MC to HC))

  13. Sample 3 Surirella Brebissonii (MC) Nitschia Sublinearis (MC to HC) Gyrosigma Nodiferum (MC)

  14. Sample 3 AFM: trace of diatom (Phase contrast)

  15. Sample 3 AFM: trace of diatom

  16. Sample 3 AFM: trace of diatom Zoom at 45 micron

  17. Sample 5

  18. Sample 7 Fragilaria Acus (SC) Fragilaria Acus (SC)

  19. Sample 7 In some cases the AFM cantilever can displace a diatom: Before: After:

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