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Phytochemical profiling of different genotypes of strawberries

Phytochemical profiling of different genotypes of strawberries. Sara Tulipani, Marche Polytechnic University Ancona, Italy. Material: List of cultivars. STORAGE Short refrigeration (3 days at 4°C + 1 day at RT). PhD Project. NUTRITIONAL QUALITY of STRAWBERRY fruits:

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Phytochemical profiling of different genotypes of strawberries

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  1. Phytochemical profiling of different genotypes of strawberries Sara Tulipani, Marche Polytechnic University Ancona, Italy

  2. Material: List of cultivars STORAGE Short refrigeration (3 days at 4°C + 1 day at RT)

  3. PhD Project NUTRITIONAL QUALITY of STRAWBERRY fruits: From the characteristics of the FRUIT to the effects on HUMAN BODY • Phytochemical and antioxidant profiling of the fruits • Studies on Bioavailability • Studies on Bioefficacy

  4. PhD Project • Phytochemical and antioxidant profiling of the fruits STSM • Studies on Bioavailability • Studies on Bioefficacy

  5. Before the STSM........ • Already available informations: • Total antioxidant capacity and vitamin C contribution • Data about total anthocyanins, total phenolics, total folate content • Preliminary studies on human volunteers

  6. STSM at PRI Institute • Moisture Content (Oven method) • Vit C content (HPLC) • Phytochemical & Antioxidant profile (HPLC coupled to online antioxidant analysis) • Additional analysis • Technical explorations

  7. 1. Moisture level (Oven method) • Range from 87.4% to 94.2%

  8. 2. Vitamin C content (HPLC) Genotype-to-genotype differences

  9. 2. Vitamin C content (HPLC) Effect of storage

  10. 3. HPLC coupled to on-line antioxidant analysis Koleva I I et al. Anal Chemistry 2001, 73, 3373-3381

  11. 3. HPLC coupled to on-line antioxidant analysis Koleva I I et al. Anal Chemistry 2001, 73, 3373-3381

  12. 3. Phenolic composition by HPLC-PDA • 254 nm: ellagic acid derivatives • 280 nm: phenolic acids • 312 nm: phenolic acids (p-coumaric acid derivatives) • 360 nm: flavonols • 512 nm: anthocyanins

  13. An example of antioxidant analysis……

  14. An example of antioxidant analysis……

  15. HPLC On-line antioxidant analysis

  16. Genotype-to-genotype differences

  17. HPLC On-line antioxidant analysis

  18. HPLC On-line antioxidant analysis

  19. Genotype-to-genotype differences RT = 13.8 p-coumaric acid glucoside RT = 14.7 p-coumaric acid glycoside M D B

  20. Genotype-to-genotype differences

  21. HPLC On-line antioxidant analysis

  22. Genotype-to-genotype differences Pg-glucoside Cy-glucoside A Pg-rutinoside Pg-malonyl glucoside G B

  23. HPLC On-line antioxidant analysis Pg-glucoside: 25 to 40% of the total antioxidant contribution (excluding vitamin C)

  24. Phytochemical composition and antioxidant contributions Effect of storage • No common trends on the variation of compounds in all clones • (No relevant effects, increase, decrease of single classes of compounds)

  25. Conclusions 9 strawberry genotypes: • Dry matter content variation • 2-fold differences on the vitamin C content • Variability on the phenolic composition on a qualitative and quantitative basis • Anthocyanins: the main contribution to TAC (vitamin C excluded) • Others polyphenols: variation on the relative antioxidant contribution

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