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結 構 生 物 學 Structural Biology

結 構 生 物 學 Structural Biology. 期 中 報 告. Speaker : Shiue-Tai Chen Adviser ：鄒文雄 老師. Crystal Structure of Human Peroxiredoxin 5, a Novel Type of Mammalian Peroxiredoxin at 1.5 Å Resolution. PDB ID ： 1HD2.

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結 構 生 物 學 Structural Biology

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  1. 結 構 生 物 學Structural Biology 期 中 報 告 Speaker : Shiue-Tai Chen Adviser ：鄒文雄 老師

  2. Crystal Structure of Human Peroxiredoxin 5, a Novel Type of Mammalian Peroxiredoxin at 1.5 Å Resolution PDB ID：1HD2 Jean-Paul Declercq,Christine Evrard,Andre Clippe et al. Journal of Molecular Biology 311(4): 751-759 2001

  3. Reactive oxygen species(ROS) are constantly generated under normal conditions as a consequence of aerobicmetabolism. • ROS include free radicals such as the superoxide anion(O2.-), the hydroxyl radical(HO.), and the nonradical hydrogen peroxide(H2O2).

  4. To cope with the deleterious effects of reactive oxygen species, aerobic organisms are equipped with antioxidant enzymes. • More than a decade ago, a novel family of peroxidases named peroxiredoxins (Prxs) emerged. (Chae,etc.1994)

  5. Topological diagram showing the arrangement of the secondary structural elements in Peroxiredoxin 5

  6. Ribbon diagrams showing the overall organization of Peroxiredoxin 5. (b) being a top view of (a).

  7. View of the surrounding of the active-site pocket. (a) Molecular surface coloured according to the local electrostatic potential,ranging from blue (the most positive region) to red (the most negative). (b) A diagram in which the side-chains of some important residues. (a) was produced using GRASP (b) using MOLSCRIPT26 and Raster3D.

  8. View of the surrounding of the active-site pocket. (c) Stereoscopic view oriented like (b) in which the electron density of a s-A28 map has been contoured at a level of 2 s around the side-chains and the benzoate ion. (c) was produced using using O.

  9. What I will do? 1.使用alignment in EBIand using GeneDoc in PSC ，為了比對PeroxiredoxinⅠ~Ⅵ胺基酸序列的特異性及保留性，與功能的關係。 2. 利用Swiss pdb Viewer軟體及網頁搜尋的PDB ID檔，來觀察PeroxiredoxinⅤ與分子內或分子間的交互作用(interaction)，與酵素活性的關係。

  10. What I will do? 3.使用上述的方法，來討論我們實驗室利用不同的物種cloning出來的Peroxiredoxin與分子生物學研究結果的相關性。

  11. Thanks you !!

  12. Prx SOD GPx O2 O2-. H2O2 H2O Catalase Fe2+ Cu+ NO. Cu2+ Fe3+ ONOO- OH. PLOOH NO2. Prx PL PHGPx PLOH

  13. Zachary A. Wood,etc.2003

  14. Zachary A. Wood,etc.2003

  15. K. Henkle-Du¨hrsen *, A. Kampko¨ tter,2001

  16. PDB ID：1HD2

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