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Microbial Detection Arrays

Microbial Detection Arrays. MiDAs. Shayla Stewart Steven To Charles Vaughan Sameera Wijesinghe. Dave Miller Elizabeth Newton Ted Schumacher. Interim Review I February 6 th , 2007 Aerospace Senior Projects University of Colorado – Boulder Advisors: Dr. Forbes and Dr. Maslanik

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Microbial Detection Arrays

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  1. Microbial Detection Arrays MiDAs Shayla Stewart Steven To Charles Vaughan Sameera Wijesinghe Dave Miller Elizabeth Newton Ted Schumacher Interim Review I February 6th, 2007 Aerospace Senior Projects University of Colorado – Boulder Advisors: Dr. Forbes and Dr. Maslanik Customers: BioServe and Tufts University

  2. Briefing Overview • Overall Design Summary • Changes Since CDR • Test Plans • Schedule • Budget • Current Issues

  3. Overall Design Summary Reagent Water Chamber Autoclaves Peristaltic Pump Mounts Top Shelf Environmental Chamber Reaction Chambers (with Mixers) NOTE: Only shows parts to be manufactured Mixer Motor Mounts

  4. Changes Since CDR • Mechanical: • Autoclave: Repositioning of screw holes and O-ring groove • Mixer: Rearrangement of impellers, addition of bottom scoop • Environmental chamber: Walls broken up to simplify manufacturing and assembly • Shelves and supports: Sizes standardized, COTS supports • Electrical: • Sensors: LM34 temperature sensors on autoclave, reagent water chamber, and ambient • PP-0079 thermistors on reaction chamber • Additional thermistors placed on reaction chambers • Software: • Laptop hard drive for data storage • Manufacturing: • BioServe manufacturing parts of autoclave bodies (milling), reaction chamber bodies, and mixers

  5. Changes Since CDR Electrical System Mixer Rectangular Mixing Blades Four Controlled Items Flat Scoop

  6. Electrical Mechanical Software Biological Integrated Test Plan Flow Chart Power Supply Circuit Reagent H2O Containment Software Input/Output Voltage Switch Board Circuit Sample Transport Integrated Electrical Subsystem Integrated System Verification Peristaltic Pump Control Circuit Mixer Control Circuit Mixing Capability Disassembly/ Reassembly Sample Sterility Autoclave Heater Autoclave Temp/Pressure Autoclave Kill Test Rxn Chamber Environ. Control TEC Control Circuit

  7. Special Test Requirements • Hazard Analyses • Electrical: electrocution/fire • Autoclave: pressure vessel (projectiles), hot surfaces, sharp edges • Assembly/Disassembly: sharp edges • Environmental control: hot surfaces • Integrated System: all of above • Testing Locations • BioServe Environmental Chamber • BioServe Wet Chemistry Lab

  8. Schedule

  9. Budget

  10. Current Issues • Tubing: • PharMed tubing does not come in 1” inner diameter • Substitution has been found (Bioprene) but has not been ordered • TEC Controller: • Power cord did not come with TEC controller • Replacement can be fabricated or purchased • Manufacturing: • Two weeks behind schedule • Offset by manufacturing contributions from BioServe • Ordering: • Pumps, thermostats, and TECs have not been ordered

  11. Conclusions • Manufacturing behind schedule • Offset by BioServe’s assistance • Most parts are ordered • Tubing is only ordering concern • Can be offset by finding other distributors • Still on budget • Over what was expected, but still under budget with most parts ordered • Mechanical, software, and electrical packages approved • Will be able to complete manufacturing and testing by deadlines

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