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Supplemental Material

This study utilizes supplemental material and quantitative mass spectrometry to assess the functional role of selected neuropeptides as regulators of feeding in rats. Annotated MS/MS spectra of peptides carrying specific post-translational modifications (PTMs) are presented, including C-term amidation, Pyro-glu formation from Q, and Pyro-glu formation from Q with amidation. A total of 120 peptides with PTMs were identified and their details are provided.

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Supplemental Material

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  1. Supplemental Material Quantitative Mass Spectrometry Reveals Food Intake-Induced Neuropeptide Level Changes in Rat Brain: Functional Assessment of Selected Neuropeptides as Feeding Regulators

  2. Figure S1. Annotated MS/MS spectra of peptides. (A) Annotated MS/MS spectra of peptides carrying PTM identified in this study. (B) Annotated MS/MS spectra of peptides that have been detected from only one biological replicate are also included. Peptides carrying C-term amidation 25 Peptides carrying Pyro-glu from Q 87 Peptides carrying Pyro-glu from Q; Amidation 8 MS/MS spectra for a total of 120 peptides with PTM as reported in this study are summarized. The details for ID are presented in the format as below.

  3. (A) Amidation 25peptides

  4. Pyro-glu from Q 87peptides

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