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Nutrient Media for Plant Tissue Cultures

Functions of medium. Provide waterProvide mineral nutritional needsProvide vitaminsProvide growth regulatorsAccess to atmosphere for gas exchangeRemoval of plant metabolite waste. Mineral Elements. Macroelements. Nitrogen (N)nitrate ion (NO3- oxidized) ammonium ion (NH4 reduced)25-60 mMorg

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Nutrient Media for Plant Tissue Cultures

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    1. Nutrient Media for Plant Tissue Cultures

    2. Functions of medium Provide water Provide mineral nutritional needs Provide vitamins Provide growth regulators Access to atmosphere for gas exchange Removal of plant metabolite waste

    3. Mineral Elements

    4. Macroelements Nitrogen (N) nitrate ion (NO3- oxidized) ammonium ion (NH4+ reduced) 25-60 mM organic

    5. Macroelements Potassium (K) 20 -30 mM Phosphorous (P) 1-3 mM Calcium (Ca) 1-3 mM Magnesium (Mg) 1-3 mM Sulfur (S) 1-3 mM

    6. Micronutrients Iron (Fe) 1 m M Manganese (Mn) 5-30 m M Zinc (Zn) Boron (B) Copper (Cu) 0.1 m M Molybdenum (Mo) 1 m M Cobalt (Co) 0.1 m M Iodine (I) Nickel (Ni), aluminum (Al), and silicon (Si)

    7. Organic Compounds Sugar sucrose others 20 to 40 g/l Vitamins thiamine (vitamin B1) nicotinic acid (niacin) and pyridoxine (B6) myo-inositol

    8. Other Compounds Activated charcoal Plant growth regulators!

    9. Support Systems Agar (from seaweed) Agarose Gelrite (Phytagel) (from bacteria) Mixtures (Phytagar) Mechanical (bridges, rafts) Sand

    10. Media Formulations Many available Differ in salt concentrations Differ in presence or absence of salts

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