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Cherry leaf roll disease

Cherry leaf roll disease. Sample Collection & Analysis. Types of samples to collect. Budsticks Collect from the end of January until bud break About 8” at the base of the 1-year-old wood One stick from each scaffold or symptomatic limb. Types of samples to collect.

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Cherry leaf roll disease

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  1. Cherry leaf roll disease Sample Collection & Analysis

  2. Types of samples to collect Budsticks • Collect from the end of January until bud break • About 8” at the base of the 1-year-old wood • One stick from each scaffold or symptomatic limb

  3. Types of samples to collect Flower & leaf buds • Bud swell to flower bud popcorn stage • Six buds in total • Collect from all major limbs and branches

  4. Types of samples to collect Leaves • Collect 2 weeks after full bloom until the end of June • Collect the first leaves that emerge in spring (oldest) • Collect six leaves in total from each tree • Collect from all major scaffold limbs including any symptomatic limbs

  5. Collect samples from all major limbs • Virus distribution is erratic, particularly with recent infections • Insure that part of each sample is collected from symptomatic shoots or from each major limb

  6. Ship samples in ziplock bags • Seal samples in ziplock bags (don’t add extra moisture) • Label bag (tree, row, grower, GPS) • Include contact information • Keep out of direct sun • Keep cool (40F), but don’t freeze • Overnight delivery to WSU ELISA Lab (early to mid-week)

  7. Shipping Information ELISA Laboratory Washington State University-IAREC 24106 N. Bunn Rd. Prosser, WA 99350 Contact: Tina Vasile ELISA Lab Manager 509-786-9382 tina_vasile@wsu.edu Ken Eastwell Project Manager 509-786-9385 keastwell@wsu.edu Ship overnight delivery via courier to: Switchboard: 509-786-9382 Facsimile: 509-786-9370

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