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Glucose Catabolism

Glucose Catabolism. Carbohydrate Metabolism. We convert all ingested carbs into glucose The breakdown of glucose generates ATP (energy) Anaerobic respiration (without oxygen) Fewer ATP Aerobic respiration (with oxygen) More ATP. This weeks activities:.

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Glucose Catabolism

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  1. Glucose Catabolism

  2. Carbohydrate Metabolism • We convert all ingested carbs into glucose • The breakdown of glucose generates ATP (energy) • Anaerobic respiration (without oxygen) • Fewer ATP • Aerobic respiration (with oxygen) • More ATP

  3. This weeks activities: • Demonstration of heat energy from cellular respiration in plants • CO2 production from yeast fermentation • Reduction reaction in one step of Kreb’s Cycle • Oxygen consumption during cellular respiration in meal worms

  4. Alcoholic Fermentation by Yeast Glucose  Ethanol + CO2 + ATP (Energy) Tube A Tube B Tube C 10 ml yeast 10 ml yeast 10 ml yeast H20 to fill glucose to fill 10 ml NaF glucose to fill

  5. Fermentation Expected Results How can you tell if fermentation occurs? (i.e. what is being measured?) In which tubes do you expect fermentation to occur? Why? Tube A Tube B Tube C 10 ml yeast 10 ml yeast 10 ml yeast H20 to fill glucose to fill 10 ml NaF glucose to fill

  6. Kreb’s cycle

  7. Experimental Reaction Using Ground Meat Hydrogen/Electron accepter Methylene Blue + H2 (colorless/pink meat) Methylene Blue (dark blue) Succinate Fumarate Succinate dehydrogenase Substrate Product Enzyme (in meat)

  8. Kreb’s Set-Up Tube #1 Tube #2 Tube #3 Tube #4 meat soln meat soln meat soln 5 drops s. acid 5 drops s. acid 5 drops s. acid 3 drops m. blue 3 drops m. blue 3 drops m. blue 3 drops m. blue 10ml NaF • 37 °C Water Bath • Observe until tube #1’s color changes from blue to clear • Remove all tubes, observe & record • Add water until half full all “meat” tubes, vortex & empty into bag • Clean tubes at sink with soap & water; rinse well

  9. Using a living organism: mealworm (larva stage of the darkling beetle)

  10. Mealworm Respiration • Measure distance Methylene blue drops moves in two tubes (in cm) • O2 consumed = difference X 0.02cm2 • Weight of all three worms in grams (1st) • O2 consumed/gm (divide O2 consumed by weight) • Resp rate = O2 consumed ÷ 0.17 hr gm

  11. Quiz 7 study guide • Understand the set-up and results from each experiment: • yeast alcoholic fermentation • succinate Kreb’s cycle • the mealworm respiration (oxygen consumption) • plant respiration demo • Read lab 8: Transporting Molecules

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