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Contents of Bioseparation

Contents of Bioseparation. Prof. In Ho Kim Chungnam National University. Chap.1 Introduction. Classification of Bioproducts Small molecules Macromolecules Proteins Nucleic acids and nucleotide Polysaccharides Engineering analysis. Chap.2 Analytical methods. Biological activity

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Contents of Bioseparation

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  1. Contents of Bioseparation Prof. In Ho Kim Chungnam National University

  2. Chap.1 Introduction • Classification of Bioproducts • Small molecules • Macromolecules • Proteins • Nucleic acids and nucleotide • Polysaccharides • Engineering analysis

  3. Chap.2 Analytical methods • Biological activity • Analysis of purity • Electrophoresis • HPLC • HPLC-Mass • Spectrophotometric assay • Microbiological assays

  4. Chap.3 Cell lysis and Flocculation • Cell structure • Prokaryotic cells • Eukaryotic cells • Cell lysis • Chemical and mechanical methods • Flocculation • Electric double layer • Flocculation rate

  5. Chap.4 Filtration • Filtration principles • Conventional and crossflow filtration • Filter media and equipment • Membrane fouling • Scaleup and design of filtration system

  6. Chap.5 Sedimentation • Equation of motion • Equilibrium sedimentation • Sedimentation coefficient • Equivalent time • Production centrifuge • Ultracentrifugation • Sedimentation at low acceleration

  7. Chap.6 Extraction • Partitioning equilibria • Phase separation • Countercurrent stage calculation • Scaleup and design of extractors

  8. Chap.7 Chromatography • Adsortion equilibrium • Column dynamics • Plate model • Rate model • Adsorbent types • Equipment • Scaleup

  9. Chap.8 Precipitation • Protein solubility • Pricipitate formation phenomena • Nucleation, growth by diffusion and fluid motion, precipitate breakage and aging • Particle size distribution • Methods of precipitation • Design of precipitation system

  10. Chap.9 Crystallization • Principles • Nucleation • Crystal growth • Kinetics • Batch crystallizers • Process crystallizers of proteins • Scaleup and design

  11. Chap.10 Drying • Principles • Water in biological solids • Heat and mass transfer • Dryer description and operation • Vacuum shelf and rotary dryer • Freeze dryer • Spray dryer • Scaleup and design

  12. Chap.11 Bioprocess design • Synthesis of bioseparation process • Primary recovery and final purification • Process analysis • Spreadsheet and simulators • Process economics • Capital and operating cost analysis • Examples(Insulin and citric acid production)

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