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Proposed Nanofactory Automated Cell to Biomolecule Analysis (ACBA) platform- coordinated by Paulaitis. 1st Stage- Cell Separation. 2nd Stage- Cell Manipulation. 3rd Stage- microRNA Detection. Sample Input. Sample Output. Cells injected into the cell track assay. Mixer.

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Sample Input

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  1. Proposed Nanofactory Automated Cell to Biomolecule Analysis (ACBA) platform- coordinated by Paulaitis 1st Stage- Cell Separation 2nd Stage- Cell Manipulation 3rd Stage- microRNA Detection Sample Input Sample Output Cells injected into the cell track assay Mixer i. Cell surface biomarkers using functionalized magnetic nanoparticles Agarwal (Paulaitis), Dorcena (Winter), Miller (Chalmers), Schley (Hansford), JLi (Liu), SSingh (Bakshi) ii. Cell migration rate using nanofiber ‘race tracking’ Blackstone (Powell), Carter (Epstein), Elmer (Palmer), Gallego-Perez (Hansford), He (Farson), Wang (Cooper), Lim (Farson), Munj (Tomasko), PSingh (Ho) i. Magnetic tweezers arrays Chen (Sooryakumar), Lee (Lu), Vieria (Sooryakumar) ii. Optical tweezer arrays LLi (Yi), Morss (Lafyatis) • iii. AFM • Nocera (Agarwal) i. Label-free single cell droplet PCR Yang (Fan) ii. Nanoelectrporation Zhao (JLee), Duong (Wyslouzil), Huang (JLee), Zhou (RLee) iii.Label-free ion transport through nanostrands Bertani (Lu), Gupta (JLee) iv. Label-free ECL in microwells Beilke (Olesik) Microfluidic flow with magnetic Nanoparticles

  2. NSEC Presentation Teams • Stage 1- Dorcena (Winter), Miller (Chalmers), Schley (Hansford); Stage 2- Chen (Sooryakumar), Jung (Lu); Stage 3- Yang (Fan) {Pouyan Boukany and Xin Hu}- 1.i/2.i/3.i on May 24 • Stage 1- Agarwal (Paulaitis), JLi (Liu), SSingh (Bakshi), Stage 2- Nocera (Agarwal); Stage 3- Beilke (Olesik), Huang (JLee) {Yun Wu and Kwang Joo Kwak}- 1.i/2.iii/3. iv on June 2 • Stage 1- Carter (Epstein), Gallego-Perez (Hansford), PSingh (Ho); Stage 2-Lee (Lu), Vieria (Sooryakumar); Stage 3- Zhao (JLee) {Sadhana Sharma and Pouyan Boukany}- 1.ii/2.i/3.ii/May 24 • Stage 1- Lim (Farson), Wang (Cooper); Stage 2- Morss (Lafyatis); Stage 3- Bertani (Lu), Gupta (JLee), Shin (Singer) {Sadhana Sharma and Bo Yu}- 1.ii/2.ii/3.iii on May 26 • Stage 1- Blackstone (Powell), Elmer (Palmer), He (Farson), Munj (Tomasko); Stage 2- LLi (Yi); Stage 3- Duong (Wyslouzil), Zhou (RLee) {Yun Wu and Lei Li}- 1.ii/2.ii/lipoplex-MB based detection on June 2

  3. NSF Request-A well developed schedule with milestones, frequent checkpoints, and metrics for ACBA • Enhanced technical metrics at the interfaces between components {e.g. how to determine the successful integration of the cell separation stage with the cell manipulation stage, before it has built the entire system?} • Realistic milestones (1-3 months) to catch and resolve expected/unexpected challenges are fleshed out in a timely manner. {e.g. intermediate milestones need to reach the 3-year goal of single cell separation/isolation is achieved} • Plans to monitor and address interfacial issues. {a system may work just right at the component level, but fails at the interfaces. Also, the system may fail as errors build up at interfaces until the total error leads to functional failure.} • How the modeling expertise to assist in both planning and executing the system integration program

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