cont

1. Supplementary Figure1. A. Immnocytochemistry of T24 cells transfected with siR-socs7 alone. Bar=20μm. B. The expression levels of STAT3 and p-STAT3 by western blotting analysis in T24 and NKB1 cells transfected with siR-socs7 alone. siRNA was transfected into cells at 20 nM. The assays were performed at 72 (T24) or 96 (NKB1) hr after the transfection. B A T24 NKB1 T24 −PIC −PIC siR-socs7 alone cont siR-socs7 cont siR-socs7 STAT3 p-STAT3 β-actin STAT3 Phalloidin Merge (siRNA; 20 nM)

2. Supplementary Figure2. A. Expression levels of IFN-β and ISGs in human melanoma A2058 cells, as determined by real time PCR. B. Protein expression levels in A2058 cells. C. Expression levels of IFN-β,ISGs, and SOCS7 in A2058 cells, as determined by real time PCR. A ** ** ** ** ** ** IFN-β OAS1 TRAIL * C B cont miR-145 −Proform PARP-1 −Cleaved form N.S * * −Proform Caspase-8 −Active form; 43 kDa −Active form; 18 kDa β-actin SOCS7 IFN-β OAS1 TRAIL